Oie H K, Easton J M, Ablashi D V, Baron S
Infect Immun. 1975 Nov;12(5):1012-7. doi: 10.1128/iai.12.5.1012-1017.1975.
Moderate amounts of viral inhibitor were produced by mouse embryo (ME) cultures infected with two strains of plaque-purified murine cytomegalovirus (MCV). This inhibitor was shown to be interferon, based on the possession of similar properties. The growth studies of MCV in ME cells showed that interferon was produced as early as 4 h after infection, infectious virus was produced between 12 to 16 h, and cytopathic effect was produced between 16 to 18 h. Since MCV-induced interferon production and the subsequent development of antiviral state occurred early, the long eclipse period may be due to an interferon-mediated delay of virus replication. Pretreatment of ME cells with varying concentrations of interferon before infection with MCV did not result in increased interferon production, but at high pretreatment doses a slight inhibitory effect on interferon production was observed. In vitro sensitivity studies showed that small doses of MCV were highly sensitive to the antiviral action of interferon, but higher viral doses proved to be markedly resistant. Although the available evidence does not permit a definitive interpretation of the mechanism by which MCV may show differing sensitivities to interferon action, the presence of a small interferon-resistant fraction of virus-infected cells may account for the observations.
用两株蚀斑纯化的鼠巨细胞病毒（MCV）感染小鼠胚胎（ME）培养物，可产生适量的病毒抑制剂。基于其具有相似的特性，该抑制剂被证明是干扰素。对ME细胞中MCV的生长研究表明，感染后4小时就产生了干扰素，12至16小时产生传染性病毒，16至18小时产生细胞病变效应。由于MCV诱导的干扰素产生以及随后抗病毒状态的发展发生得较早，较长的隐蔽期可能是由于干扰素介导的病毒复制延迟。在感染MCV之前，用不同浓度的干扰素预处理ME细胞不会导致干扰素产生增加，但在高预处理剂量下，观察到对干扰素产生有轻微抑制作用。体外敏感性研究表明，小剂量的MCV对干扰素的抗病毒作用高度敏感，但较高的病毒剂量则表现出明显的抗性。尽管现有证据不允许对MCV对干扰素作用可能表现出不同敏感性的机制进行明确解释，但病毒感染细胞中一小部分对干扰素耐药的部分可能可以解释这些观察结果。
