Laso Francisco Javier, Vaquero José Miguel, Almeida Julia, Marcos Miguel, Orfao Alberto
Unidad de Alcoholismo, Servicio de Medicina Interna II, Hospital Universitario, Salamanca, Spain.
Cytometry B Clin Cytom. 2007 Sep;72(5):408-15. doi: 10.1002/cyto.b.20169.
Controversial results have been reported about the effects of alcoholism on the functionality of monocytes. In the present study we analyze the effects of chronic alcoholism on the intracellular production of inflammatory cytokines by peripheral blood (PB) monocytes.
Spontaneous and in vitro-stimulated production of interleukin (IL) 1alpha (TNFalpha) by PB monocytes was analyzed at the single level by flow cytometry in chronic alcoholics without liver disease and active ethanol (EtOH) intake (AWLD group), as well as in patients with alcohol liver cirrhosis (ALC group), who were either actively drinking (ALCET group) or with alcohol withdrawal (ALCAW group).
A significantly increased spontaneous production of IL1beta, IL6, IL12, and TNFalpha was observed on PB monocytes among AWLD individuals. Conversely, circulating monocytes form ALCET patients showed an abnormally low spontaneous and stimulated production of inflammatory cytokines. No significant changes were observed in ALCAW group as regards production of IL1beta, IL6, IL12, and TNFalpha.
Our results show an altered pattern of production of inflammatory cytokines in PB monocytes from chronic alcoholic patients, the exact abnormalities observed depending on both the status of EtOH intake and the existence of alcoholic liver disease.
关于酒精中毒对单核细胞功能的影响，已有相互矛盾的研究结果报道。在本研究中，我们分析了慢性酒精中毒对外周血（PB）单核细胞炎性细胞因子胞内产生的影响。
采用流式细胞术，在单细胞水平分析无肝病且有乙醇（EtOH）摄入的慢性酒精中毒者（无肝病且有乙醇摄入组）以及酒精性肝硬化患者（酒精性肝硬化组）外周血单核细胞白细胞介素（IL）-1α（肿瘤坏死因子α）的自发产生及体外刺激产生情况，酒精性肝硬化组患者分为仍在饮酒者（酒精性肝硬化仍在饮酒组）和已戒酒者（酒精性肝硬化已戒酒组）。
在无肝病且有乙醇摄入组个体的外周血单核细胞中，观察到IL-1β、IL-6、IL-12和肿瘤坏死因子α的自发产生显著增加。相反，酒精性肝硬化仍在饮酒组患者循环单核细胞的炎性细胞因子自发产生和刺激产生均异常低下。酒精性肝硬化已戒酒组在IL-1β、IL-6、IL-12和肿瘤坏死因子α产生方面未观察到显著变化。
我们的结果显示，慢性酒精中毒患者外周血单核细胞炎性细胞因子产生模式发生改变，具体异常情况取决于乙醇摄入状态和酒精性肝病的存在与否。
