Michael N L, Vahey M, Burke D S, Redfield R R
Department of Retroviral Research, Walter Reed Army Institute of Research, Rockville, Maryland 20850.
J Virol. 1992 Jan;66(1):310-6. doi: 10.1128/JVI.66.1.310-316.1992.
Studies of cultivatable human immunodeficiency virus type 1 (HIV-1) from plasma samples from infected patients have shown a correspondence between increasing viral burden and disease progression, but these measurements are selective and thus nonrepresentative of the in vivo viral load. Quantitation of proviral DNA sequences by the polymerase chain reaction in purified CD4+ T cells has shown a similar relationship but does not provide a measure of viral gene expression. We have studied viral DNA, genomic RNA, and spliced mRNA expression of HIV-1 in infected patients with a quantitative polymerase chain reaction assay. Viral RNA expression is detected in all stages of infection. These data show that the natural history of HIV infection is associated with a shift in the balance of viral expression favoring the production of genomic RNA without a preceding period of true viral latency.
对来自感染患者血浆样本中可培养的1型人类免疫缺陷病毒（HIV-1）的研究表明，病毒载量增加与疾病进展之间存在对应关系，但这些测量具有选择性，因此不能代表体内病毒载量。通过聚合酶链反应对纯化的CD4+T细胞中的前病毒DNA序列进行定量分析，显示出类似的关系，但无法提供病毒基因表达的测量方法。我们使用定量聚合酶链反应分析法研究了感染患者体内HIV-1的病毒DNA、基因组RNA和剪接mRNA的表达情况。在感染的所有阶段都能检测到病毒RNA表达。这些数据表明，HIV感染的自然史与病毒表达平衡的转变有关，这种转变有利于基因组RNA的产生，而不存在真正的病毒潜伏期。
