Thomas B, Gilchrist C, Taylor-Robinson D
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex, UK.
Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):659-62. doi: 10.1007/BF01975821.
During a study of women with laparoscopically investigated pelvic pain, genital tract specimens were examined for Chlamydia trachomatis using a direct fluorescent antibody (DFA) technique (MicroTrak, Syva) and culture. Some smears, particularly those from the upper genital tract, contained an inadequate number of cells when examined by the DFA technique and many cell monolayers were destroyed by the specimens. To obtain results for such samples, or to confirm the original DFA result, additional specimens which had been frozen at -70 degrees C or in liquid nitrogen were centrifuged at high speed and the resulting deposit examined by the DFA technique. By this means, 32 negative results were confirmed for specimens from 10 chlamydia-negative patients with pelvic inflammatory disease or with high chlamydial antibody titres, and 26 negative results were confirmed for 19 patients who were positive at other sites. In addition, three chlamydia-positive and six chlamydia-negative results were obtained for sites where the original smear for DFA testing had been inadequate (few epithelial cells) and six specimens that were negative originally were found to be positive. Thus, of 73 specimens that were either inadequate or negative by DFA testing originally, 9 (12%) were positive by DFA testing after centrifugation.
在一项针对腹腔镜检查有盆腔疼痛的女性的研究中，使用直接荧光抗体（DFA）技术（MicroTrak，Syva公司）和培养法对生殖道标本进行沙眼衣原体检测。一些涂片，尤其是来自上生殖道的涂片，用DFA技术检查时细胞数量不足，许多细胞单层被标本破坏。为了获得此类样本的结果，或确认原始DFA结果，将保存在-70℃或液氮中的额外标本进行高速离心，所得沉淀物用DFA技术检查。通过这种方法，对10例患有盆腔炎或衣原体抗体滴度高但衣原体检测阴性的患者的标本，32个阴性结果得到确认；对其他部位呈阳性的19例患者，26个阴性结果得到确认。此外，对于DFA检测原始涂片不合格（上皮细胞少）的部位，获得了3个衣原体阳性结果和6个衣原体阴性结果，并且发现6个原本阴性的标本呈阳性。因此，在最初DFA检测不合格或呈阴性的73个标本中，9个（12%）经离心后DFA检测呈阳性。
