Thomas B J, MacLeod E J, Hay P E, Horner P J, Taylor-Robinson D
MRC Sexually Transmitted Diseases Research Group, St. Mary's Hospital, London, UK.
Eur J Clin Microbiol Infect Dis. 1994 Aug;13(8):651-5. doi: 10.1007/BF01973991.
Enzyme immunoassays (EIAs) are widely used to diagnose chlamydial infections in patients attending genitourinary medicine clinics. They are relatively easy to perform and are suitable for testing large numbers of samples. The objective of this study was to determine what proportion of women with chlamydial infection, defined as the presence of Chlamydia trachomatis in a cervical smear or deposit and/or in the urinary tract, detected by means of a sensitive direct fluorescent antibody test could also be identified by using two commercially available EIAs to test cervical samples. On hundred fifty-one women attending the genitourinary medicine clinic at St. Mary's Hospital, London, were enrolled. The use of the Chlamydiazyme (Abbott Diagnostics, UK) and MicroTrak (Syva, UK) EIAs resulted in the identification of only 56% and 63%, respectively, of women with chlamydial infection detected by direct fluorescent antibody staining. Thus, the EIAs available for detection of chlamydiae in cervical samples are inadequate for identifying all infected women. Improvement might be achieved by testing multiple samples or by resorting to tests of greater sensitivity.
酶免疫测定法（EIA）被广泛用于诊断就诊于泌尿生殖医学诊所的患者的衣原体感染。它们相对易于操作，适合检测大量样本。本研究的目的是确定通过敏感的直接荧光抗体试验检测出的衣原体感染女性（定义为宫颈涂片或沉积物和/或尿道中存在沙眼衣原体）中，有多大比例也可以通过使用两种市售的EIA检测宫颈样本而被识别出来。招募了151名在伦敦圣玛丽医院泌尿生殖医学诊所就诊的女性。使用衣原体酶（英国雅培诊断公司）和微追踪（英国赛瓦公司）EIA分别仅识别出通过直接荧光抗体染色检测出的衣原体感染女性的56%和63%。因此，用于检测宫颈样本中衣原体的EIA不足以识别所有感染女性。通过检测多个样本或采用更高灵敏度的检测方法可能会有所改进。
