使用单个纳米孔进行溶液中的单分子质谱分析。
Single-molecule mass spectrometry in solution using a solitary nanopore.
作者信息
Robertson Joseph W F, Rodrigues Claudio G, Stanford Vincent M, Rubinson Kenneth A, Krasilnikov Oleg V, Kasianowicz John J
机构信息
Electronics and Electrical Engineering Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA.
出版信息
Proc Natl Acad Sci U S A. 2007 May 15;104(20):8207-11. doi: 10.1073/pnas.0611085104. Epub 2007 May 9.
Abstract
We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single alpha-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.
摘要
我们介绍了一种基于纳米级孔隙与分析物之间相互作用的二维溶液质谱方法。例如，进入单个α-溶血素孔隙的聚乙二醇分子会导致具有特征性平均停留时间的明显质量依赖性电导状态。基于电导的质谱能够清晰分辨乙二醇的重复单元，且平均停留时间随聚乙二醇质量单调增加。该技术可能对溶液中分子的实时表征有用。
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