Khan Saifullah, Khan Gul Majid
Department of Pharmaceutics, Faculty of Pharmacy, Gomal University, Dera Ismail Khan (NWFP) Pakistan.
Pak J Pharm Sci. 2007 Oct;20(4):279-84.
Rhazya stricta is a small glabrous shrub, widely distributed throughout Western Asia from Yemen to Arabia, to the North West Province of India and abundantly found in various regions of Pakistan. Larvicidal and antifungal studies of polar and non polar aerial parts extracts of Rhazya stricta were performed using brine shrimps larvae for larvicidal study and for antifungal study microorganisms, Trichophyton longifusis, Aspergillus flavus, Candida albicans, Microsporum canis and Fusarium solani were used respectively. The methanol fraction showed significant cytotoxicity with LC50 17.809 microg/ml, having mortality rate 73.33% at highest dose. While pet-ether, chloroform and carbon tetrachloride possessed moderate to low cytotoxicity with their LC50 values 49.077 microg/ml, 95.859 microg/ml and 80.489 microg/ml respectively, ethyl acetate fraction showed no cytotoxicity. Results of antifungal studies showed that fractionated samples of methanol and chloroform possessed significant antifungal activities against, Trichophyton longifusis, Aspergillus flavus, Candida albicans and Fusarium solani respectively. Due to these promising results, further in vivo studies over R. stricta must be conducted.
锐药柽柳是一种小型无毛灌木，广泛分布于从也门到阿拉伯半岛的整个西亚地区，直至印度西北省份，在巴基斯坦的各个地区也大量存在。使用卤虫幼虫进行了锐药柽柳极性和非极性地上部分提取物的杀幼虫研究，并分别使用须癣毛癣菌、黄曲霉、白色念珠菌、犬小孢子菌和茄病镰刀菌进行抗真菌研究。甲醇提取物显示出显著的细胞毒性，LC50为17.809微克/毫升，在最高剂量下死亡率为73.33%。而石油醚、氯仿和四氯化碳的细胞毒性为中度至低度，其LC50值分别为49.077微克/毫升、95.859微克/毫升和80.489微克/毫升，乙酸乙酯提取物未显示细胞毒性。抗真菌研究结果表明，甲醇和氯仿的分级样品分别对须癣毛癣菌、黄曲霉、白色念珠菌和茄病镰刀菌具有显著的抗真菌活性。由于这些有前景的结果，必须对锐药柽柳进行进一步的体内研究。
