Lee Kiyoung, Choo Yoe-Jin, Giovannoni Stephen J, Cho Jang-Cheon
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1815-1818. doi: 10.1099/ijs.0.65032-0.
Gram-negative, facultatively aerobic, chemoheterotrophic, short rod-shaped marine bacterial strains HTCC2662(T) and HTCC2663, isolated from the Sargasso Sea by using a dilution-to-extinction culturing method, were investigated to determine their taxonomic position. Characterization of the two strains by phenotypic and phylogenetic analyses revealed that they belonged to the same species. The DNA G+C content of strain HTCC2662(T) was 58.4 mol% and the predominant cellular fatty acids were C(18 : 1) omega 7c (52.5 %), C(16 : 0) 2-OH (13.5 %) and C(18 : 1) 11-methyl omega 7c (12.2 %). Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains represented a distinct line of descent within the genus Ruegeria, with highest sequence similarities to Ruegeria atlantica DSM 5823(T) (97.2 %), Ruegeria lacuscaerulensis DSM 11314(T) (96.5 %) and Ruegeria pomeroyi DSM 15171(T) (95.6 %). Several phenotypic characteristics, including facultatively requiring NaCl and oxygen for growth, together with the cellular fatty acid composition, differentiated strain HTCC2662(T) from other members of the genus Ruegeria. Based on phenotypic, chemotaxonomic and phylogenetic traits, it is suggested that strains HTCC2662(T) and HTCC2663 represent a novel species of the genus Ruegeria, for which the name Ruegeria pelagia sp. nov. is proposed. The type strain is HTCC2662(T) (=KCCM 42378(T)=NBRC 102038(T)).
采用稀释至灭绝培养法从马尾藻海分离得到革兰氏阴性、兼性需氧、化能异养、短杆状海洋细菌菌株HTCC2662(T)和HTCC2663，对其分类地位进行了研究。通过表型和系统发育分析对这两个菌株进行表征，结果表明它们属于同一物种。菌株HTCC2662(T)的DNA G+C含量为58.4 mol%，主要细胞脂肪酸为C(18 : 1) ω7c（52.5%）、C(16 : 0) 2-OH（13.5%）和C(18 : 1) 11-甲基ω7c（12.2%）。16S rRNA基因序列的系统发育分析表明，这些菌株代表了鲁杰氏菌属内一个独特的谱系，与大西洋鲁杰氏菌DSM 5823(T)（97.2%）、蓝湖鲁杰氏菌DSM 11314(T)（96.5%）和波默罗伊鲁杰氏菌DSM 15171(T)（95.6%）的序列相似性最高。包括兼性需要NaCl和氧气才能生长在内的几个表型特征，以及细胞脂肪酸组成，使菌株HTCC2662(T)与鲁杰氏菌属的其他成员区分开来。基于表型、化学分类和系统发育特征，建议菌株HTCC2662(T)和HTCC2663代表鲁杰氏菌属的一个新物种，为此提出新名称鲁杰氏浮游菌（Ruegeria pelagia sp. nov.）。模式菌株为HTCC2662(T)（=KCCM 42378(T)=NBRC 102038(T)）。
