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从猿猴病毒40中分离出的带切口DNA-蛋白质复合物的结构：蛋白质与DNA的共价连接及切口特异性

Structure of a nicked DNA-protein complex isolated from simian virus 40: covalent attachment of the protein to DNA and nick specificity.

作者信息

Kasamatsu H, Wu M

出版信息

Proc Natl Acad Sci U S A. 1976 Jun;73(6):1945-9. doi: 10.1073/pnas.73.6.1945.

DOI:10.1073/pnas.73.6.1945
PMID:180531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430424/
Abstract

A portion of the nicked circular DNA isolated from purified simian virus 40 contains a protein-DNA complex in which protein(s) is covalently attached to the end of a DNA single strand. (Nicked DNA is double-stranded DNA that contains at least one single-strand scission.) The protein was visualized by electron microscopy and labeled in vitro with 125I. The bond between the protein and the DNA is stable in alkali, 4 M guanidine-hydrochloride, 3.86 M hydroxylamine (pH 4,23), and in 98% formamide. Most of the molecules in the nicked circular DNA fraction contained one nick. The nick occurs on either of the two complementary strands; the specific nick sites on the two strands are staggered, but lie within a few hundred nucleotides of each other.

摘要

从纯化的猿猴病毒40中分离出的带切口环状DNA的一部分含有一种蛋白质-DNA复合物，其中蛋白质与DNA单链末端共价连接。（带切口的DNA是含有至少一个单链断裂的双链DNA。）通过电子显微镜观察到该蛋白质，并在体外用125I进行标记。蛋白质与DNA之间的键在碱、4M盐酸胍、3.86M羟胺（pH 4.23）和98%甲酰胺中稳定。带切口环状DNA部分中的大多数分子含有一个切口。切口出现在两条互补链中的任意一条上；两条链上的特定切口位点是错开的，但彼此相距几百个核苷酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60a/430424/cdceff63883d/pnas00036-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60a/430424/35dfb5929579/pnas00036-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60a/430424/cdceff63883d/pnas00036-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60a/430424/35dfb5929579/pnas00036-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f60a/430424/cdceff63883d/pnas00036-0182-a.jpg

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Structure of a nicked DNA-protein complex isolated from simian virus 40: covalent attachment of the protein to DNA and nick specificity.从猿猴病毒40中分离出的带切口DNA-蛋白质复合物的结构：蛋白质与DNA的共价连接及切口特异性
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