Erdogan H M, Karapehlivan M, Citil M, Atakisi O, Uzlu E, Unver A
Department of Internal Diseases, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey.
Vet Res Commun. 2008 Apr;32(4):333-9. doi: 10.1007/s11259-008-9036-z. Epub 2008 Feb 5.
This study was designed to disclose some indicators of oxidative stress and inflammation in natural cases of bovine leptospirosis. For this purpose, 12 bulls exhibiting clinical signs of leptospirosis and 10 healthy bulls were used. Animals were subjected to thorough clinical examination and the clinical signs were recorded. All animals were blood sampled in order to determine serum total sialic acid (TSA), lipid bound sialic acid (LBSA), malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), uric acid (UA), total protein (TP), albumin and glucose. Urine samples were collected from each animal and examined under dark-field microscope to observe spirochetes. Diseased animals exhibited clinical signs suggesting leptospirosis and the diagnosis was supported by positive dark-field microscope examination. Mean TSA (mmol/L), LBSA (mmol/L), TP (g/dl), albumin (g/dl), glucose (mg/dl), MDA (micromol/L), GSH (mg/dl), NO (nmol/ml), and UA (mg/L) levels were 1.63 +/- 0.02, 0.40 +/- 0.10, 7.18 +/- 0.24, 3.23 +/- 0.5, 64.96 +/- 1.88, 5.71 +/- 0.11, 78.68 +/- 0.72, 7.94 +/- 0.34, and 8.75 +/- 0.41 in healthy bulls, and 2.50 +/- 0.05, 0.70 +/- 0.2, 9.27 +/- 0.17, 2.55 +/- 0.62, 107.93 +/- 2.52, 8.82 +/- 0.14, 47.85 +/- 1.85, 14.57 +/- 0.63 and 15.85 +/- 0.80 in leptospirosis cases, respectively. The differences between the two groups were statistically significant (P < 0.001). Increased TSA, LBSA, MDA, NO, UA, TP, glucose and decreased GSH and albumin concentrations were suggestive of inflammation and oxidative stress in diseased bulls. The results obtained may suggest that oxidative damage along with other mechanisms might have taken part in the pathogenesis of bovine leptospirosis and further detailed studies are needed to fully understand the mechanism(s) of the disease.
本研究旨在揭示牛钩端螺旋体病自然病例中的一些氧化应激和炎症指标。为此，使用了12头表现出钩端螺旋体病临床症状的公牛和10头健康公牛。对动物进行了全面的临床检查并记录了临床症状。采集所有动物的血液以测定血清总唾液酸（TSA）、脂质结合唾液酸（LBSA）、丙二醛（MDA）、还原型谷胱甘肽（GSH）、一氧化氮（NO）、尿酸（UA）、总蛋白（TP）、白蛋白和葡萄糖。从每只动物收集尿液样本并在暗视野显微镜下检查以观察螺旋体。患病动物表现出提示钩端螺旋体病的临床症状，暗视野显微镜检查阳性支持了诊断。健康公牛的平均TSA（mmol/L）、LBSA（mmol/L）、TP（g/dl）、白蛋白（g/dl）、葡萄糖（mg/dl）、MDA（micromol/L）、GSH（mg/dl）、NO（nmol/ml）和UA（mg/L）水平分别为1.63±0.02、0.40±0.10、7.18±0.24、3.23±0.5、64.96±1.88、5.71±0.11、78.68±0.72、7.94±0.34和8.75±0.41，而钩端螺旋体病病例中分别为2.50±0.05、0.70±0.2、9.27±0.17、2.55±0.62、107.93±2.52、8.82±0.14、47.85±1.85、14.57±0.63和15.85±0.80。两组之间的差异具有统计学意义（P<0.001）。TSA、LBSA、MDA、NO、UA、TP、葡萄糖升高以及GSH和白蛋白浓度降低提示患病公牛存在炎症和氧化应激。获得的结果可能表明氧化损伤与其他机制可能参与了牛钩端螺旋体病的发病机制，需要进一步详细研究以充分了解该疾病的机制。
