Kundu Joydip, Dewan Moumita, Ghoshal Sarani, Kundu S C
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India.
J Mater Sci Mater Med. 2008 Jul;19(7):2679-89. doi: 10.1007/s10856-008-3398-1. Epub 2008 Feb 19.
Silk fibroin from silk gland of Bombyx mori 5th instar larvae was utilized to fabricate films, which may find possible applications as two-dimensional matrices for tissue engineering. Bombyx mori cocoon fibroin is well characterized as potential biomaterial by virtue of its good mechanical strength, water stability, thermal properties, surface roughness and biocompatibility. The present study aims to characterize the biophysical, thermal, mechanical, rheological, swelling properties along with spectroscopic analysis, surface morphology and biocompatibility of the silk gland fibroin films compared with cocoon fibroin. Fibroin solutions showed increased turbidity and shear thinning at higher concentration. The films after methanol treatment swelled moderately and were less hydrophilic compared to the untreated. The spectroscopic analysis of the films illustrated the presence of various amide peaks and conformational transition from random coil to beta sheet on methanol treatment. X-ray diffraction studies also confirmed the secondary structure. Thermogravimetric analysis showed distinct weight loss of the films. The films were mechanically stronger and AFM studies showed surfaces were rougher on methanol treatment. The matrices were biocompatible and supported L929 mouse fibroblast cell growth and proliferation. The results substantiate the silk gland fibroin films as potential biomaterial matrices.
利用家蚕五龄幼虫丝腺中的丝素蛋白制备薄膜，该薄膜有望作为组织工程中的二维基质得到应用。家蚕茧丝素蛋白凭借其良好的机械强度、水稳定性、热性能、表面粗糙度和生物相容性，被充分表征为潜在的生物材料。本研究旨在表征丝腺丝素蛋白薄膜与茧丝素蛋白相比的生物物理、热、机械、流变、溶胀性能，以及光谱分析、表面形态和生物相容性。丝素蛋白溶液在较高浓度下浊度增加且呈现剪切变稀现象。与未处理的薄膜相比，经甲醇处理后的薄膜适度溶胀且亲水性较低。薄膜的光谱分析表明存在各种酰胺峰，且经甲醇处理后从无规卷曲向β-折叠发生构象转变。X射线衍射研究也证实了二级结构。热重分析显示薄膜有明显的重量损失。薄膜机械强度更高，原子力显微镜研究表明经甲醇处理后表面更粗糙。该基质具有生物相容性，支持L929小鼠成纤维细胞的生长和增殖。结果证实丝腺丝素蛋白薄膜是潜在的生物材料基质。
