Mansour I, Doinel C, Rouger P
Institut National de Transfusion Sanguine, Paris, France.
Clin Exp Immunol. 1991 Sep;85(3):481-4. doi: 10.1111/j.1365-2249.1991.tb05753.x.
In order to highlight the underlying mechanism(s) of the CD8 lymphocyte expansion in the HIV infection, two distinct CD8 subsets were analysed: T CD8bright+ CD3+ with MHC-restricted activity, and non-T CD8dim+ CD3-, which performs natural killer (NK) activity. It consists of a cross-sectional study including 168 HIV-infected patients (74 CDC stage II, 48 CDC stage III and 46 CDC stage IV) compared among them and to 60 healthy individuals. We observed an expansion of CD8+ CD3+ cells which masks a depletion of CD8+ CD3-. The comparative study showed that the expansion of the CD8+ CD3+ is relatively higher than that of total CD8+ lymphocytes and that the depletion of the CD8+ CD3- subset is severe, begins early and remains constant through the HIV progression. The comparison of CD4/CD8 and CD4/CD8+ CD3+ ratios showed that the latter could possibly be a better indicator in the HIV infection. The mechanism of inverted CD4/CD8 ratio in healthy individuals was also clarified. The CD8+ CD3+, CD8+ CD3- and CD4/CD8+ CD3+ parameters would be more specific markers than total CD8 and CD4/CD8 ratio especially in therapy trials.
为了突出HIV感染中CD8淋巴细胞扩增的潜在机制，分析了两个不同的CD8亚群：具有MHC限制活性的T CD8bright+ CD3+，以及具有自然杀伤（NK）活性的非T CD8dim+ CD3-。这是一项横断面研究，纳入了168例HIV感染患者（74例CDC II期、48例CDC III期和46例CDC IV期），对他们之间以及与60名健康个体进行比较。我们观察到CD8+ CD3+细胞的扩增掩盖了CD8+ CD3-的减少。比较研究表明，CD8+ CD3+的扩增相对高于总CD8+淋巴细胞的扩增，并且CD8+ CD3-亚群的减少很严重，在HIV病程早期开始并持续存在。CD4/CD8和CD4/CD8+ CD3+比值的比较表明，后者可能是HIV感染中更好的指标。健康个体中CD4/CD8比值倒置的机制也得到了阐明。CD8+ CD3+、CD8+ CD3-和CD4/CD8+ CD3+参数将比总CD8和CD4/CD8比值更具特异性，尤其是在治疗试验中。
