Bukhman V M, Treshchalina E M, Krasnopol'skaia L M, Isakova E B, Sedakova L A, Avtonomova A V, Leont'eva M I, Soboleva N Iu, Belitskiĭ I V, Bakanov A V
Antibiot Khimioter. 2007;52(1-2):4-9.
The basidiomycetes Ganoderma lucidum, Hericium erinaceus, Lentinus edodes and Trametes versicolor were used for preparation of aqueous extracts. A polysaccharide preparation (VPG) was isolated from the G. lucidum aqueous extracts. The mycelium was grown under submerged conditions according to an original procedure. Preliminary exposure of mice with tumors to cyclosphosphamide in a low dose for prolonged elimination of T-suppressors and rapid recovery of T-killers induced an increase in the efficacy of the H. erinaceus and L. edodes extracts. Investigation of the aqueous extracts and VPG on different tumor strain lines for the potential Modifiers of Biological Response (Ca755, s/c P388, s-180) demonstrated antitumor activity and satisfactory tolerabily after oral administration. Inhibition of the tumor growth by the H. erinaceus and T. versicolor extracts and VPG amounted to 88-99% and that of s-180 treated with the L. edodes aqueous extract amounted to 66-75%. Compositions 1, 2, 4 amd 5 were significantly more active by the duration and value of the effect on the animal tumor nodes as compared to the aqueous extracts and VPG included to the compositions and composition 4. Composition 5 (T. versicolor + H. erinaceus + G. Lucidum) proved to be the most efficient by all the criteria. The results of the design of the technologies for cultivation of the mycelum of the medicinal basidiomycetes, investigation of the antumor properties of the extracts and polysaccharide fraction of the mycelium and development of efficient compositions on their basis are summarized. Composition 5 proved to be the most promising for the clinical trials.
担子菌纲的灵芝、猴头菇、香菇和云芝用于制备水提取物。从灵芝水提取物中分离出一种多糖制剂（VPG）。菌丝体按照原始程序在深层培养条件下生长。预先用低剂量环磷酰胺处理荷瘤小鼠，以长期消除T抑制细胞并使T杀伤细胞快速恢复，可提高猴头菇和香菇提取物的疗效。对不同肿瘤细胞系（Ca755、s/c P388、s-180）的水提取物和VPG进行生物反应潜在调节剂的研究，结果表明口服给药后具有抗肿瘤活性且耐受性良好。猴头菇和云芝提取物以及VPG对肿瘤生长的抑制率达88-99%，香菇水提取物处理的s-180肿瘤生长抑制率达66-75%。与组合物中包含的水提取物和VPG以及组合物4相比，组合物1、2、4和5在对动物肿瘤结节的作用持续时间和效果值方面活性明显更高。组合物5（云芝+猴头菇+灵芝）在所有标准下都被证明是最有效的。总结了药用担子菌菌丝体培养技术的设计结果、菌丝体提取物和多糖组分的抗肿瘤特性研究以及在此基础上开发高效组合物的情况。组合物5被证明最有希望用于临床试验。
