Chan Kwok Hung, Yam Wing Cheong, Pang Chiu Mei, Chan Kit Man, Lam Siu Yan, Lo Kam Fai, Poon Leo L M, Peiris J S Malik
Department of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong, SAR, People's Republic of China.
J Clin Microbiol. 2008 Jul;46(7):2195-9. doi: 10.1128/JCM.00315-08. Epub 2008 May 7.
The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.
对NucliSens easyMAG和BioRot 9604自动化核酸提取系统进行了评估,并与手动QIAamp(Qiagen)提取方法进行比较,以考察它们从鼻咽抽吸物样本中提取核酸用于检测RNA和DNA呼吸道病毒的能力。三种方法回收的核酸在PCR检测中具有相当的灵敏度,且三种方法得到的病毒载量相当。没有证据表明存在残留的PCR抑制剂,也没有PCR交叉污染的证据。