Liu Hong-Wei, Li Jian-Kuan, Zhang Da-Wei, Zhang Jing-Chao, Wang Nai-Li, Cai Guo-Ping, Yao Xin-Sheng
School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, China.
J Asian Nat Prod Res. 2008 May-Jun;10(5-6):521-9. doi: 10.1080/10286020801966674.
Two new sulfated steroidal compounds (1 and 2), along with three known steroidal saponins (3, 4, and 5) were isolated from the starfish Asterias amurensis Lutken. The structures of new compounds were elucidated as 3beta-O-sulfated-cholest-5-ene-7alpha-ol (1) and (E) 25-O-beta-d-xylopyranosyl-26, 27-dinor-24(S)-methyl-22-ene-15alpha-O-sulfated-5alpha-cholest-3beta,6alpha-ol (2) by extensive NMR experiments and chemical evidence. Their effects on UMR106 cell proliferation were screened by MTT method. The results indicated that compounds 2 and 2a (0.01-100 microM) significantly promoted the osteoblastic proliferation. The initial structure-activity relationship analysis suggests that the sugar moiety is the necessary group for the activity.
从多棘海盘车（Asterias amurensis Lutken）中分离出两种新的硫酸化甾体化合物（1和2）以及三种已知的甾体皂苷（3、4和5）。通过广泛的核磁共振实验和化学证据，确定新化合物的结构分别为3β - O - 硫酸化胆甾 - 5 - 烯 - 7α - 醇（1）和（E）25 - O - β - D - 吡喃木糖基 - 26,27 - 二降 - 24（S） - 甲基 - 22 - 烯 - 15α - O - 硫酸化 - 5α - 胆甾 - 3β,6α - 醇（2）。采用MTT法筛选它们对UMR106细胞增殖的影响。结果表明，化合物2和2a（0.01 - 100微摩尔）显著促进成骨细胞增殖。初步的构效关系分析表明，糖部分是该活性的必需基团。
