Allahyari Saeideh, Delazar Abbas, Najafi Moslem
Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2014 Dec;4(Suppl 2):577-82. doi: 10.5681/apb.2014.085. Epub 2014 Dec 31.
This study was aimed to evaluate general toxicity, anti-oxidant activity and effects of Ficus carica leaves extract on ischemia/reperfusion injuries.
Antioxidant activity, total phenolic and flavonoid compounds of 70% methanolic extract of Ficus carica leaves were measured. The general toxicity test was carried out by brine shrimp lethality assay. Isolated hearts of male rats were mounted on a Langendorff apparatus and perfused with modified Krebs-Henseleit solution. In control group, the hearts were perfused with normal Krebs solution, however, treatment groups received enriched solution with the extract (0.04, 0.2 and 1 mg/ml) during stabilization and reperfusion (after 30 min global ischemia), respectively. Cardiac arrhythmias were analyzed and TTC method was used for infarct size determination.
The extract displayed antioxidant activity in the DPPH assay (RC50=0.06666 mg/ml). Total phenolic content was 12.29 mg GAE/100 g dry sample and the amount of flavonoids was calculated 40.729 mg/g. LD50 value by brine shrimp test was 0.158 mg/ml. The extract decreased number of VEBs, incidence and duration of Rev VF with clear reduction in infarct size and infarct volume (P<0.001).
Ficus carica decreased ischemia/reperfusion-induced injuries. These protections are probably due to antioxidant capacity and the existence of flavonoid and phenolic compounds in the extract.
本研究旨在评估无花果叶提取物的一般毒性、抗氧化活性及其对缺血/再灌注损伤的影响。
测定无花果叶70%甲醇提取物的抗氧化活性、总酚和黄酮类化合物含量。通过卤虫致死试验进行一般毒性测试。将雄性大鼠的离体心脏安装在Langendorff装置上，并用改良的Krebs-Henseleit溶液灌注。对照组心脏用正常Krebs溶液灌注，然而，治疗组在稳定期和再灌注期（30分钟全心缺血后）分别接受含提取物（0.04、0.2和1mg/ml）的富集溶液。分析心律失常情况，并采用TTC法测定梗死面积。
提取物在DPPH试验中显示出抗氧化活性（RC50 = 0.06666mg/ml）。总酚含量为12.29mg GAE/100g干样品，黄酮类化合物含量计算为40.729mg/g。卤虫试验的LD50值为0.158mg/ml。提取物减少了室性早搏的数量、室性心动过速的发生率和持续时间，梗死面积和梗死体积明显减小（P<0.001）。
无花果叶可减轻缺血/再灌注诱导的损伤。这些保护作用可能归因于提取物的抗氧化能力以及黄酮类和酚类化合物的存在。
