Patel H, Frenkel L D, Greenhalgh M, Howell R, Patel S
Department of Pediatrics, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick 08903-0019.
J Clin Microbiol. 1991 Feb;29(2):410-2. doi: 10.1128/jcm.29.2.410-412.1991.
Rapid confirmation of herpes simplex virus (HSV) is essential in many clinical settings. Viral isolation in cell culture is the standard method for diagnosing HSV infection, with confirmation by specific immunological staining. The performance of the Rapid Absorbent Matrix Pad (RAMP) HSV culture confirmation test was evaluated with specimens obtained from 71 patients with suspected HSV infection and inoculated into African green monkey kidney cell lines. Forty-one culture-positive specimens were confirmed to be HSV by both the RAMP HSV test and the Bartels HSV immunoperoxidase test. Thirty immunoperoxidase-negative specimens were also negative in the RAMP HSV test. The sensitivity and specificity of the RAMP HSV test were 100%. Twelve specimens positive for cytomegalovirus, adenovirus, or enterovirus tested negative by the RAMP HSV test. Thus, the RAMP HSV test was faster than, easier to perform than, as sensitive as, and as specific as other well-documented confirmation methods.
在许多临床情况下，快速确诊单纯疱疹病毒（HSV）至关重要。细胞培养中的病毒分离是诊断HSV感染的标准方法，并通过特异性免疫染色进行确认。使用从71例疑似HSV感染患者获取的标本接种到非洲绿猴肾细胞系中，对快速吸收基质垫（RAMP）HSV培养确认试验的性能进行了评估。41份培养阳性标本通过RAMP HSV试验和巴特尔HSV免疫过氧化物酶试验均确认为HSV。30份免疫过氧化物酶阴性标本在RAMP HSV试验中也为阴性。RAMP HSV试验的敏感性和特异性均为100%。12份巨细胞病毒、腺病毒或肠道病毒阳性的标本经RAMP HSV试验检测为阴性。因此，RAMP HSV试验比其他有充分文献记载的确认方法更快、更易于操作，且敏感性和特异性相同。
