利用A549细胞在离心培养中通过快速膜酶免疫测定法检测单纯疱疹病毒
Detection of herpes simplex virus by using A549 cells in centrifugation culture with a rapid membrane enzyme immunoassay.
作者信息
Hursh D A, Wendt S F, Lee C F, Gleaves C A
机构信息
Diagnostic Virology Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
出版信息
J Clin Microbiol. 1989 Jul;27(7):1695-6. doi: 10.1128/jcm.27.7.1695-1696.1989.
Abstract
The RAMP herpes simplex virus (HSV) culture confirmation test was compared with immunofluorescence (IF) staining with a specific HSV monoclonal antibody reagent for the detection of HSV in centrifugation culture. The RAMP test detected 47 of 57 IF-positive specimens (sensitivity, 88.6%) and agreed with 217 of 220 IF-negative specimens (specificity, 98.6%). The RAMP test can be performed in less than 15 min and gives an immediate visual result. However, the sensitivity and the false-positive and false-negative results need further investigation.
摘要
将RAMP单纯疱疹病毒（HSV）培养确认试验与使用特异性HSV单克隆抗体试剂的免疫荧光（IF）染色法进行比较，以检测离心培养物中的HSV。RAMP试验在57份IF阳性标本中检测出47份（灵敏度为88.6%），并与220份IF阴性标本中的217份结果一致（特异性为98.6%）。RAMP试验可在不到15分钟内完成，并能立即给出可视化结果。然而，其灵敏度以及假阳性和假阴性结果仍需进一步研究。
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本文引用的文献
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Laboratory diagnosis of herpes simplex virus infections. Principles guiding the development of rapid diagnostic tests.单纯疱疹病毒感染的实验室诊断。指导快速诊断检测方法开发的原则。
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