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采用柯达SureCell疱疹检测法检测单纯疱疹病毒。

Detection of herpes simplex virus by the Kodak SureCell Herpes Test.

作者信息

Dorian K J, Beatty E, Atterbury K E

机构信息

Columbus Health Department, Ohio 43215.

出版信息

J Clin Microbiol. 1990 Sep;28(9):2117-9. doi: 10.1128/jcm.28.9.2117-2119.1990.

DOI:10.1128/jcm.28.9.2117-2119.1990
PMID:2172300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268116/
Abstract

The Kodak SureCell Herpes Test Kit (SC-HSV) was evaluated in a high-risk sexually transmitted disease clinic population. A total of 97 lesion specimens (from 94 patients) were tested for the presence of herpes simplex virus by SC-HSV and by spin-amplified tissue culture confirmed by an enzyme immunoassay. The overall sensitivity of SC-HSV compared with that of spin-amplified tissue culture-enzyme immunoassay was 81.1%; the specificity was 100%. For vesicular lesions only, SC-HSV sensitivity was 100%; for nonvesicular lesions, sensitivity was 75.6%, SC-HSV was rapid (less than 15 min) and very easy to perform.

摘要

柯达SureCell疱疹检测试剂盒(SC-HSV)在一个高风险性传播疾病门诊人群中进行了评估。通过SC-HSV以及经酶免疫测定确认的旋转扩增组织培养法,对总共97份(来自94名患者的)皮损标本进行了单纯疱疹病毒检测。与旋转扩增组织培养-酶免疫测定法相比,SC-HSV的总体灵敏度为81.1%;特异性为100%。仅对于水疱性皮损,SC-HSV的灵敏度为100%;对于非水疱性皮损,灵敏度为75.6%。SC-HSV检测速度快(少于15分钟)且操作非常简便。

相似文献

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Detection of herpes simplex virus by the Kodak SureCell Herpes Test.采用柯达SureCell疱疹检测法检测单纯疱疹病毒。
J Clin Microbiol. 1990 Sep;28(9):2117-9. doi: 10.1128/jcm.28.9.2117-2119.1990.
2
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Comparison of three monoclonal antibody-based enzyme immunoassays for detection of herpes simplex virus in clinical specimens.三种基于单克隆抗体的酶免疫测定法检测临床标本中单纯疱疹病毒的比较
Eur J Clin Microbiol Infect Dis. 1995 Apr;14(4):314-7. doi: 10.1007/BF02116524.
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J Clin Microbiol. 1993 Jul;31(7):1774-6. doi: 10.1128/jcm.31.7.1774-1776.1993.
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Detection of herpes simplex virus by a nonradiometric spin-amplified in situ hybridization assay.通过非放射性自旋扩增原位杂交试验检测单纯疱疹病毒
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引用本文的文献

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Opportunities for rapid viral diagnosis.快速病毒诊断的机会。
Clin Microbiol Newsl. 1993 May 1;15(9):65-69. doi: 10.1016/0196-4399(93)90015-F. Epub 2002 Nov 14.
2
Physical and chemical methods for enhancing rapid detection of viruses and other agents.用于加强病毒及其他病原体快速检测的物理和化学方法。
Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150.
3
Comparison of three monoclonal antibody-based enzyme immunoassays for detection of herpes simplex virus in clinical specimens.三种基于单克隆抗体的酶免疫测定法检测临床标本中单纯疱疹病毒的比较
Eur J Clin Microbiol Infect Dis. 1995 Apr;14(4):314-7. doi: 10.1007/BF02116524.
4
Evaluation of a visual, rapid, membrane enzyme immunoassay for the detection of herpes simplex virus antigen.用于检测单纯疱疹病毒抗原的可视化快速膜酶免疫测定法的评估
J Clin Microbiol. 1991 Apr;29(4):842-5. doi: 10.1128/jcm.29.4.842-845.1991.

本文引用的文献

1
Genital herpes simplex virus infections: clinical manifestations, course, and complications.生殖器单纯疱疹病毒感染:临床表现、病程及并发症
Ann Intern Med. 1983 Jun;98(6):958-72. doi: 10.7326/0003-4819-98-6-958.
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Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.病毒分离、直接免疫荧光法和间接免疫过氧化物酶技术检测生殖器单纯疱疹病毒感染的比较
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Rapid diagnosis of herpes simplex virus infections by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法快速诊断单纯疱疹病毒感染
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Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen.用于检测单纯疱疹病毒抗原的酶联免疫吸附测定法的评估
J Clin Microbiol. 1984 Jun;19(6):730-2. doi: 10.1128/jcm.19.6.730-732.1984.
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Centrifugation-shell vial technique for rapid detection of herpes simplex virus cytopathic effect in Vero cells.用于在Vero细胞中快速检测单纯疱疹病毒细胞病变效应的离心管瓶技术
J Clin Microbiol. 1987 Feb;25(2):423-4. doi: 10.1128/jcm.25.2.423-424.1987.
6
Diagnosis of herpes simplex virus by direct immunofluorescence and viral isolation from samples of external genital lesions in a high-prevalence population.通过直接免疫荧光法和从高流行人群的外生殖器病变样本中进行病毒分离来诊断单纯疱疹病毒。
J Clin Microbiol. 1987 Feb;25(2):323-6. doi: 10.1128/jcm.25.2.323-326.1987.
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Enzyme-linked immunosorbent assay spin amplification technique for herpes simplex virus antigen detection.用于单纯疱疹病毒抗原检测的酶联免疫吸附测定自旋放大技术
J Clin Microbiol. 1986 Aug;24(2):310-1. doi: 10.1128/jcm.24.2.310-311.1986.
8
Comparison of the detection of herpes simplex virus in direct clinical specimens with herpes simplex virus-specific DNA probes and monoclonal antibodies.运用单纯疱疹病毒特异性DNA探针和单克隆抗体对直接临床标本中的单纯疱疹病毒检测进行比较。
J Clin Microbiol. 1985 Nov;22(5):748-53. doi: 10.1128/jcm.22.5.748-753.1985.
9
Epidemiology of genital herpes simplex virus infection.单纯疱疹病毒生殖器感染的流行病学
Epidemiol Rev. 1985;7:127-46. doi: 10.1093/oxfordjournals.epirev.a036279.
10
Detection of herpes simplex virus DNA from genital lesions by in situ hybridization.通过原位杂交检测生殖器病变中的单纯疱疹病毒DNA。
J Clin Microbiol. 1988 May;26(5):933-7. doi: 10.1128/jcm.26.5.933-937.1988.