Doucet-Populaire F, Trieu-Cuot P, Dosbaa I, Andremont A, Courvalin P
Laboratoire d'Ecologie Microbienne, Institut Gustave-Roussy, Villejuif, France.
Antimicrob Agents Chemother. 1991 Jan;35(1):185-7. doi: 10.1128/AAC.35.1.185.
Transfer of conjugative transposon Tn1545 from Enterococcus faecalis to Listeria monocytogenes was studied in vitro and in vivo. Tn1545 transferred following filter mating at a frequency of 2.5 x 10(-7) transconjugants per donor colony. A 20-fold increase in transfer frequency was observed when matings were performed in the presence of a subinhibitory concentration of tetracycline. The frequency of in vivo transfer of Tn1545, expressed as the number of transconjugants per donor cell extracted from the intestines of the gnotobiotic mice after 35 days of experiment, was 1.1 x 10(-8). Presence of a low concentration of tetracycline in the drinking water increased this frequency 10-fold.
在体外和体内研究了接合转座子Tn1545从粪肠球菌向单核细胞增生李斯特菌的转移情况。通过滤膜交配，Tn1545的转移频率为每个供体菌落产生2.5×10⁻⁷个接合子。当在亚抑制浓度的四环素存在下进行交配时，转移频率增加了20倍。以实验35天后从悉生小鼠肠道中提取的每个供体细胞产生的接合子数量表示，Tn1545的体内转移频率为1.1×10⁻⁸。饮用水中低浓度四环素的存在使该频率增加了10倍。
