Frisby D, Eaton M, Fellner P
Nucleic Acids Res. 1976 Oct;3(10):2771-87. doi: 10.1093/nar/3.10.2771.
The nature of the 5' terminus of encephalomyocarditis (EMC) virion RNA has been investigated. We have failed to detect any capped products or nucleoside polyphosphates arising upon complete digestion of the RNA with T1, T2, and pancreatic ribonucleases, and it would therefore appear that the 5' terminus of EMC virus RNA is not phosphorylated and not capped with m7G. EMC virions do contain, however, large amounts of all four 5'-monosubstituted nucleoside triphosphates (4.2M pppG; 16.4M pppA; 3.OM pppU and 2.5M pppC), of which at least a proportion (about 15-20%) appear to remain bound to fully denatured RNA in the presence of divalent cations.
对脑心肌炎（EMC）病毒粒子RNA的5'末端性质进行了研究。在用T1、T2和胰核糖核酸酶完全消化RNA后，我们未能检测到任何加帽产物或核苷多磷酸盐，因此EMC病毒RNA的5'末端似乎未被磷酸化且未被m7G加帽。然而，EMC病毒粒子确实含有大量的四种5'-单取代核苷三磷酸（4.2M pppG；16.4M pppA；3.0M pppU和2.5M pppC），其中至少一部分（约15-20%）在二价阳离子存在下似乎仍与完全变性的RNA结合。
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