Colmenares C, Sutrave P, Hughes S H, Stavnezer E
Department of Molecular Genetics, University of Cincinnati Medical Center, Ohio 45267-0524.
J Virol. 1991 Sep;65(9):4929-35. doi: 10.1128/JVI.65.9.4929-4935.1991.
The v-ski oncogene is a truncated version of the cellular proto-oncogene, c-ski, and lacks sequences coding for both the N- and C-terminal ends of the c-ski protein. In the region of overlap, v-ski and c-ski differ by only one amino acid. To determine whether these differences underlie v-ski's oncogenic activation, we have cloned cDNAs for several alternatively spliced c-ski mRNAs and introduced these cDNAs into replication-competent retroviral vectors. The biological activities of these c-ski constructs have been compared with those of v-ski. We found that all c-ski gene products, when expressed at high levels from the promoter in the retroviral long terminal repeat, can induce morphological transformation, anchorage independence, and muscle differentiation in avian cells. Cells that are susceptible to ski-induced transformation and myogenesis normally express endogenous c-ski at low levels. Thus, it appears that overexpression of ski is sufficient for oncogenic and myogenic activation.
v-ski癌基因是细胞原癌基因c-ski的截短版本，缺少编码c-ski蛋白N端和C端的序列。在重叠区域，v-ski和c-ski仅相差一个氨基酸。为了确定这些差异是否是v-ski致癌激活的基础，我们克隆了几种选择性剪接的c-ski mRNA的cDNA，并将这些cDNA导入具有复制能力的逆转录病毒载体中。已将这些c-ski构建体的生物学活性与v-ski的生物学活性进行了比较。我们发现，当从逆转录病毒长末端重复序列中的启动子高水平表达时，所有c-ski基因产物均可诱导禽类细胞发生形态转化、不依赖贴壁生长和肌肉分化。易受ski诱导的转化和肌生成影响的细胞通常低水平表达内源性c-ski。因此，似乎ski的过表达足以实现致癌和肌生成激活。
