Departamento Sanidad Animal, Facultad de Veterinaria, UCM, Madrid, Spain.
Med Mycol. 2009;47(6):601-8. doi: 10.1080/13693780802380537.
The possible co-existence of different genotypes of Aspergillus fumigatus in the same case was studied in five patients colonized or infected by this opportunistic mould. A total of 22 isolates were typed by random amplified polymorphic DNA (RAPD) and microsatellite analysis. Differences in the mating type and invasiveness of the isolates were also considered. The combination of four arbitrary primers used in RAPD typing differentiated all the isolates. In microsatellite analysis, at least two different genotypes were identified in four of the five patients. The 22 isolates showed elastase activity after 10 days of incubation at 37 degrees C in an elastin-containing medium. The presence of strains of compatible mating type was observed in one of the colonized patients and one of the individuals with invasive aspergillosis. Some isolates that belonged to the same genotype in microsatellite analysis were of a different mating type. Taken together, our results suggest that multiple isolates of A. fumigatus obtained from colonized or infected patients may differ not only in their genotypes, but also in their invasiveness and mating types. Furthermore, mating type determination may be of great assistance in differentiating some isolates, as two isolates of different mating type cannot be genotypically identical.
本研究旨在探讨同一病例中不同基因型烟曲霉的可能共存。对 5 例被这种机会性霉菌定植或感染的患者的 22 株分离株进行了随机扩增多态性 DNA（RAPD）和微卫星分析。还考虑了分离株交配型和侵袭性的差异。RAPD 分型中使用的 4 个任意引物的组合可区分所有分离株。在微卫星分析中，5 例患者中有 4 例至少鉴定出两种不同的基因型。22 株分离株在含有弹性蛋白的培养基中于 37°C 孵育 10 天后显示弹性蛋白酶活性。在定植患者和侵袭性曲霉菌病患者中均观察到了相容交配型菌株的存在。在微卫星分析中属于同一基因型的一些分离株的交配型不同。综上所述，我们的结果表明，从定植或感染患者中获得的多个烟曲霉分离株不仅在基因型上存在差异，而且在侵袭性和交配型上也存在差异。此外，交配型的确定可能有助于区分一些分离株，因为不同交配型的两个分离株不能具有相同的基因型。
