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1
Escherichia coli orfE (upstream of pyrE) encodes RNase PH.大肠杆菌的orfE（位于pyrE上游）编码核糖核酸酶PH。
J Bacteriol. 1991 Sep;173(17):5589-91. doi: 10.1128/jb.173.17.5589-5591.1991.
2
Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli.大肠杆菌orfE/rph基因产物核糖核酸酶PH的过表达与快速纯化
J Biol Chem. 1992 Aug 25;267(24):17147-52.
3
Molecular and mutational analysis of three genes preceding pyrE on the Escherichia coli chromosome.大肠杆菌染色体上pyrE之前三个基因的分子与突变分析。
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Identification of the rph (RNase PH) gene of Bacillus subtilis: evidence for suppression of cold-sensitive mutations in Escherichia coli.枯草芽孢杆菌rph（核糖核酸酶PH）基因的鉴定：大肠杆菌中冷敏感突变抑制的证据。
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Characterization of Escherichia coli RNase PH.大肠杆菌核糖核酸酶PH的特性分析
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The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.铜绿假单胞菌pyrE-crc-rph区域的核苷酸序列及crc基因产物的纯化
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Cloning and sequencing of an Escherichia coli K12 gene which encodes a polypeptide having similarity to the human ferritin H subunit.编码与人类铁蛋白H亚基具有相似性的多肽的大肠杆菌K12基因的克隆与测序。
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Crit Rev Biochem Mol Biol. 2019 Jun;54(3):242-300. doi: 10.1080/10409238.2019.1651816.
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Regulation of Bacterial Gene Expression by Transcription Attenuation.转录衰减调控细菌基因表达。
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Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding.来自枯草芽孢杆菌的磷酸解外切核糖核酸酶RNase PH的晶体结构及其对四级结构和tRNA结合的影响
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7
Identification of the rph (RNase PH) gene of Bacillus subtilis: evidence for suppression of cold-sensitive mutations in Escherichia coli.枯草芽孢杆菌rph（核糖核酸酶PH）基因的鉴定：大肠杆菌中冷敏感突变抑制的证据。
J Bacteriol. 1992 Jul;174(14):4727-35. doi: 10.1128/jb.174.14.4727-4735.1992.
8
The presence of only one of five exoribonucleases is sufficient to support the growth of Escherichia coli.五种外切核糖核酸酶中仅有一种的存在就足以支持大肠杆菌的生长。
J Bacteriol. 1992 Oct;174(20):6682-4. doi: 10.1128/jb.174.20.6682-6684.1992.

本文引用的文献

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Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG).大肠杆菌核糖体蛋白L28（rpmB）和L33（rpmG）基因的克隆及核苷酸序列
Mol Gen Genet. 1981;184(2):218-23. doi: 10.1007/BF00272908.
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A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
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Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region.大肠杆菌pyrE基因及蛋白质编码区前DNA的核苷酸序列。
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The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
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Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attentuation.大肠杆菌pyrE操纵子的结构以及由UTP调节的顺反子间衰减对pyrE表达的控制
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Mechanism of UTP-modulated attenuation at the pyrE gene of Escherichia coli: an example of operon polarity control through the coupling of translation to transcription.UTP调节大肠杆菌pyrE基因衰减的机制：通过翻译与转录偶联进行操纵子极性控制的一个实例
EMBO J. 1984 Dec 1;3(12):2857-61. doi: 10.1002/j.1460-2075.1984.tb02220.x.
8
3' processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement.核糖核酸酶缺陷型大肠杆菌中tRNA前体的3' 加工。体外加工系统的建立与特性以及对磷酸盐需求的证据
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The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.完整大肠杆菌染色体的物理图谱：一种用于大型基因组文库快速分析和分类的新策略的应用
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Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation.利用pyrE衰减子探测转录与翻译的偶联，检测大肠杆菌中密码子定义的核糖体暂停。
Nucleic Acids Res. 1985 Jun 11;13(11):4113-23. doi: 10.1093/nar/13.11.4113.

大肠杆菌的orfE（位于pyrE上游）编码核糖核酸酶PH。

Escherichia coli orfE (upstream of pyrE) encodes RNase PH.

作者信息

Ost K A, Deutscher M P

机构信息

Department of Biochemistry, University of Connecuticut Health Center, Farmington 06030.

出版信息

J Bacteriol. 1991 Sep;173(17):5589-91. doi: 10.1128/jb.173.17.5589-5591.1991.

DOI:10.1128/jb.173.17.5589-5591.1991
PMID:1885537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC208279/
Abstract

RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.

摘要

从大肠杆菌提取物中纯化出的核糖核酸酶PH至同质状态，并对其进行氨基末端测序。将该序列与基因库数据库中的所有开放阅读框进行比较，结果显示与大肠杆菌染色体上81.7分钟处pyrE上游一个未鉴定的开放阅读框（orfE）至少有95%的同一性。orfE的克隆过度表达核糖核酸酶PH活性，证实orfE编码这种核糖核酸酶。我们建议将orfE重新命名为rph。