Buñag R D, Teräväinen T L
Department of Pharmacology, College of Health Sciences and Hospital, University of Kansas Medical Center, Kansas City 66103.
Mech Ageing Dev. 1991 Jun 14;59(1-2):197-213. doi: 10.1016/0047-6374(91)90085-e.
To identify rat strains suitable for studying age-related development of hypertension we compared pressures measured with the tail-cuff method in different groups of ageing Fischer 344, Wistar, or Sprague-Dawley rats. Preliminary experiments to establish optimal frequency of chronic blood pressure measurement in ageing rats showed that tail-cuff systolic pressures did not differ significantly whether taken weekly or monthly. Repeated tail-cuff measurements were comparable even when a common cuff size was used in different groups of rats with varying tail diameters. Additional studies were then carried out in 1-year old male Wistar and Sprague-Dawley rats to measure tail-cuff pressures monthly during the second year of age. Systolic and mean pressures increased progressively with age in both strains, as did body weight and heart rate, but the incidence of hypertension was higher in Sprague-Dawley than in Wistar rats. Elevations in mean pressures were sometimes more pronounced than those in systolic pressure. Two months after the last tail-cuff measurement, the presence of hypertension in Wistar rats was verified by the elevated mean pressures that were recorded from femoral artery catheters. Our results overall suggest that the predisposition to hypertension was higher in Sprague-Dawley than in Wistar or Fischer 344 rats of the same age, and also in males than in females of the same strain. Of all the different strains and sexes we compared, therefore, male Sprague-Dawley rats from 20 to 24 months of age may be the best model for studying the development of systolic hypertension with age.
为了确定适合研究高血压年龄相关性发展的大鼠品系，我们比较了不同组衰老的Fischer 344、Wistar或Sprague-Dawley大鼠用尾套法测量的血压。在衰老大鼠中建立慢性血压测量最佳频率的初步实验表明，无论是每周还是每月测量，尾套收缩压均无显著差异。即使在不同尾径的大鼠组中使用相同尺寸的尾套，重复测量的尾套血压仍具有可比性。随后，对1岁雄性Wistar和Sprague-Dawley大鼠进行了额外研究，在其2岁期间每月测量尾套血压。两种品系的收缩压和平均压均随年龄增长而逐渐升高，体重和心率也是如此，但Sprague-Dawley大鼠的高血压发生率高于Wistar大鼠。平均压的升高有时比收缩压更为明显。在最后一次尾套测量后两个月，通过股动脉导管记录的升高的平均压证实了Wistar大鼠存在高血压。我们的研究结果总体表明，同年龄的Sprague-Dawley大鼠比Wistar或Fischer 344大鼠更容易患高血压，且同品系的雄性大鼠比雌性大鼠更容易患高血压。因此，在我们比较的所有不同品系和性别中，20至24月龄的雄性Sprague-Dawley大鼠可能是研究收缩期高血压随年龄发展的最佳模型。
