dos Reis Samara Bonesso, de Oliveira Caroline Candida, Acedo Simone Coghetto, Miranda Daniel Duarte da Conceição, Ribeiro Marcelo Lima, Pedrazzoli José, Gambero Alessandra
Clinical Pharmacology and Gastroenterology Unit, São Francisco University Medical School, Bragança Paulista, SP, Brazil.
Phytother Res. 2009 Mar;23(3):324-9. doi: 10.1002/ptr.2626.
Inflammatory bowel disease (IBD), Crohn's disease and ulcerative colitis are chronic enteropathies that probably result from a dysregulated mucosal immune response. These pathologies are characterized by oxidative and nitrosative stress, leukocyte infiltration and up-regulation of pro-inflammatory substances. Current IBD treatment presents limitations in both efficacy and safety that stimulated the search for new active compounds. Garcinia cambogia extract has attracted interest due to its pharmacological properties, including gastroprotective effects. In this study, the antiinflammatory activity of a garcinia extract was assessed in TNBS-induced colitis rats. The results obtained revealed that garcinia administration to colitic rats significantly improved the macroscopic damage and caused substantial reductions in increases in MPO activity, COX-2 and iNOS expression. In addition, garcinia extract treatment was able to reduce PGE(2) and IL-1beta colonic levels. These antiinflammatory actions could be related to a reduction in DNA damage in isolated colonocytes, observed with the comet assay. Finally, garcinia extract caused neither mortality nor toxicity signals after oral administration. As such, the antiinflammatory effects provided by the Garcinia cambogia extract result in an improvement of several parameters analysed in experimental colitis and could provide a source for the search for new antiinflammatory compounds useful in IBD treatment.
炎症性肠病（IBD）、克罗恩病和溃疡性结肠炎是慢性肠道疾病，可能是由黏膜免疫反应失调引起的。这些病症的特征是氧化应激和亚硝化应激、白细胞浸润以及促炎物质的上调。目前IBD的治疗在疗效和安全性方面都存在局限性，这促使人们寻找新的活性化合物。藤黄果提取物因其药理特性，包括胃保护作用，而引起了人们的关注。在本研究中，在三硝基苯磺酸（TNBS）诱导的结肠炎大鼠中评估了藤黄果提取物的抗炎活性。获得的结果显示，给结肠炎大鼠施用藤黄果可显著改善宏观损伤，并使髓过氧化物酶（MPO）活性、环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）表达的增加大幅降低。此外，藤黄果提取物治疗能够降低结肠中前列腺素E2（PGE2）和白细胞介素-1β（IL-1β）的水平。这些抗炎作用可能与彗星试验中观察到的分离结肠细胞中DNA损伤的减少有关。最后，藤黄果提取物口服后既未引起死亡也未出现毒性信号。因此，藤黄果提取物提供的抗炎作用导致实验性结肠炎中分析的几个参数得到改善，并可能为寻找可用于IBD治疗的新抗炎化合物提供一个来源。
