Makhlouf Houssine, Gmitro Arthur F, Tanbakuchi Anthony A, Udovich Josh A, Rouse Andrew R
University of Arizona, Department of Radiology, P. O. Box 245067, Tucson, Arizona 85724-5067, USA.
J Biomed Opt. 2008 Jul-Aug;13(4):044016. doi: 10.1117/1.2950313.
We describe the design and operation of a multispectral confocal microendoscope. This fiber-based fluorescence imaging system consists of a slit-scan confocal microscope coupled to an imaging catheter that is designed to be minimally invasive and allow for cellular level imaging in vivo. The system can operate in two imaging modes. The grayscale mode of operation provides high resolution real-time in vivo images showing the intensity of fluorescent signal from the specimen. The multispectral mode of operation uses a prism as a dispersive element to collect a full multispectral image of the fluorescence emission. The instrument can switch back and forth nearly instantaneously between the two imaging modes (less than half a second). In the current configuration, the multispectral confocal microendoscope achieves 3-microm lateral resolution and 30-microm axial resolution. The system records light from 500 to 750 nm, and the minimum resolvable wavelength difference varies from 2.9 to 8.3 nm over this spectral range. Grayscale and multispectral imaging results from ex-vivo human tissues and small animal tissues are presented.
我们描述了一种多光谱共聚焦微型内窥镜的设计与操作。这种基于光纤的荧光成像系统由一个狭缝扫描共聚焦显微镜与一个成像导管相连组成，该成像导管设计为微创型，可在体内进行细胞水平成像。该系统可在两种成像模式下运行。灰度操作模式提供高分辨率的实时体内图像，显示来自样本的荧光信号强度。多光谱操作模式使用棱镜作为色散元件来收集荧光发射的完整多光谱图像。该仪器可在两种成像模式之间几乎瞬间切换（不到半秒）。在当前配置下，多光谱共聚焦微型内窥镜实现了3微米的横向分辨率和30微米的轴向分辨率。该系统记录500至750纳米的光，在此光谱范围内，最小可分辨波长差从2.9纳米到8.3纳米不等。文中展示了来自离体人体组织和小动物组织的灰度及多光谱成像结果。
