Zhang Keyun, Liu Hui, Sun Jie, Liu Jingrui, Fei Kan, Zhang Chongxing, Xu Mingxu, Sun Jing, Ma Xiyuan, Lai Ren, Wu Yidong, Lin Maosong
Department of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Key laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China. Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.
J Nematol. 2008 Jun;40(2):127-37.
The genetic diversity and phylogeny of 26 isolates of Bursaphelenchus xylophilus from China, Japan, Portugal and North America were investigated based on the D2/3 domain of 28S rDNA, nuclear ribosomal Internal Transcribed Spacer (ITS) sequences, and random amplified polymorphic DNA (RAPD) analysis. The genetic diversity analysis showed that the D2/3 domain of 28S rDNA of isolates of B. xylophilus from China, Portugal, Japan and the US were identical and differed at one to three nucleotides compared to those from Canada. ITS sequences of isolates from China and Portugal were the same; they differed at one or two nucleotides compared to those of Japanese isolates and at four and 23 nucleotides compared to those from the US and Canada, respectively. The phylogenetic analysis indicated that Chinese isolates share a common ancestor with one of the two Japanese clades and that the Canadian isolates form a sister group of the clade comprised of isolates from China, Portugal, Japan, and the US. The relationship between Japanese isolates and those from China was closer than with the American isolates. The Canadian isolates were the basal group of B. xylophilus. This suggests that B. xylophilus originated in North America and that the B. xylophilus that occurs in China could have been first introduced from Japan. Further analysis based on RAPD analysis revealed that the relationship among isolates from Guangdong, Zhejiang, Shandong, Anhui provinces and Nanjing was the closest, which suggests that pine wilt disease in these Chinese locales was probably dispersed from Nanjing, where this disease first occurred in China.
基于28S rDNA的D2/3结构域、核糖体DNA内转录间隔区（ITS）序列以及随机扩增多态性DNA（RAPD）分析，对来自中国、日本、葡萄牙和北美的26株松材线虫分离株的遗传多样性和系统发育进行了研究。遗传多样性分析表明，来自中国、葡萄牙、日本和美国的松材线虫分离株的28S rDNA的D2/3结构域相同，与来自加拿大的分离株相比，在1至3个核苷酸处存在差异。来自中国和葡萄牙的分离株的ITS序列相同；与日本分离株相比，它们在1或2个核苷酸处存在差异，与美国和加拿大的分离株相比，分别在4和23个核苷酸处存在差异。系统发育分析表明，中国分离株与两个日本分支之一具有共同祖先，加拿大分离株形成了由来自中国、葡萄牙、日本和美国的分离株组成的分支的姐妹群。日本分离株与中国分离株的关系比与美国分离株的关系更密切。加拿大分离株是松材线虫的基部类群。这表明松材线虫起源于北美，中国的松材线虫可能最初是从日本引入的。基于RAPD分析的进一步分析表明，来自广东、浙江、山东、安徽等省以及南京的分离株之间的关系最为密切，这表明中国这些地区的松树萎蔫病可能是从该病在中国首次发生的南京传播而来的。
