Zhang Xiaopeng, Liang Zian, Siddiqui Zafar Ali, Gong Yuhua, Yu Ziniu, Chen Shouwen
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
J Ind Microbiol Biotechnol. 2009 Jun;36(6):815-20. doi: 10.1007/s10295-009-0556-5. Epub 2009 Apr 1.
Spodoptera exigua is one of the most renowned agricultural pest insects and relatively insensitive to Bacillus thuringiensis subsp. kurstaki strains which are widely used commercial products to control lepidopterans such as Heliothis armigera. In the current study, we have developed a new and efficient approach to screen and breed a B. thuringiensis subsp. kurstaki strain exhibiting high toxicity against S. exigua while retaining its high toxicity against H. armigera. UV and diethyl sulfate methods were used for mutagenesis, followed by an agar plug plate diffusion assay for preliminary screening of Zwittermicin A over-producing mutants, from which we obtained a mutant strain, designated here as B. thuringiensis subsp. kurstaki D1-23, with high toxicity against S. exigua. The toxicity of D1-23 against S. exigua and H. armigera was improved by 115.4 and 25.9%, respectively, compared to its parental commercial strain BMB005.
甜菜夜蛾是最著名的农业害虫之一，对苏云金芽孢杆菌库尔斯塔克亚种菌株相对不敏感，而这些菌株是广泛用于防治棉铃虫等鳞翅目害虫的商业产品。在本研究中，我们开发了一种新的高效方法，用于筛选和培育对甜菜夜蛾具有高毒性同时对棉铃虫仍保持高毒性的苏云金芽孢杆菌库尔斯塔克亚种菌株。采用紫外线和硫酸二乙酯方法进行诱变，随后通过琼脂块平板扩散试验对过产生物胺菌素A的突变体进行初步筛选，从中获得了一个对甜菜夜蛾具有高毒性的突变菌株，在此命名为苏云金芽孢杆菌库尔斯塔克亚种D1-23。与亲本商业菌株BMB005相比，D1-23对甜菜夜蛾和棉铃虫的毒性分别提高了115.4%和25.9%。
