Harrison Michael, Murphy Ronan P, O'Connor Paul L, O'Gorman Donal J, McCaffrey Noel, Cummins Philip M, Moyna Niall M
Department of Health, Sport and Exercise Science, Waterford Institute of Technology, Waterford, Ireland.
Eur J Appl Physiol. 2009 Jul;106(4):555-62. doi: 10.1007/s00421-009-1050-5. Epub 2009 Apr 5.
Triglyceride-rich postprandial lipoproteins are known to activate endothelial cells in vitro, contributing to atherosclerosis. Endothelial microparticles (EMP) are membranous vesicles released into the circulation from vascular endothelial cells that permit cell activation to be monitored in vivo. The objective of the study was to examine changes in EMP following a high fat meal, consumed with and without prior exercise. Eight recreationally active young men underwent two oral fat tolerance tests following either 100 min exercise at 70% VO(2)peak (EX trial) or no exercise (CON trial) on the previous evening. Postprandial triglycerides were reduced (1.97 +/- 0.31 vs. 1.17 +/- 0.13 mmol L(-1), p < 0.05) and HDL-cholesterol (HDL-C) increased (1.20 +/- 0.07 vs. 1.30 +/- 0.08 mmol L(-1), p < 0.05) in the EX compared to CON trial. EMP (CD31+/42b-) increased postprandially (p < 0.05). However, counts were not different between trials (postprandial CON and EX trial counts x 10(3 )microL(-1), 3.10 +/- 0.14 vs. 3.26 +/- 0.37). There were no changes in sICAM-1 or sVCAM-1 postprandially and no differences between trials. Interleukin-6 (IL-6) and leukocytes increased postprandially (p < 0.05). IL-6 values were not different between trials. Leukocytes were higher at 0 h in the EX trial with CON and EX trial values similar at 6 h. EMP, but not sICAM-1 or sVCAM-1, increase in response to a high fat meal. However, EMP are not attenuated by acute exercise, despite a considerable reduction in postprandial lipemia and an increase in HDL-C.
富含甘油三酯的餐后脂蛋白在体外可激活内皮细胞，促进动脉粥样硬化。内皮微粒（EMP）是从血管内皮细胞释放到循环中的膜性小泡，可用于在体内监测细胞激活情况。本研究的目的是检测高脂餐后EMP的变化，高脂餐分别在运动前和运动后食用。8名有休闲运动习惯的年轻男性在前一晚进行了两次口服脂肪耐量试验，一次是在70%VO₂峰值下运动100分钟后（运动试验），另一次是未运动（对照试验）。与对照试验相比，运动试验餐后甘油三酯降低（1.97±0.31对1.17±0.13 mmol/L⁻¹，p<0.05），高密度脂蛋白胆固醇（HDL-C）升高（1.20±0.07对1.30±0.08 mmol/L⁻¹，p<0.05）。餐后EMP（CD31⁺/42b⁻）增加（p<0.05）。然而，试验之间的计数没有差异（餐后对照试验和运动试验计数×10³μL⁻¹，3.10±0.14对3.26±0.37）。餐后可溶性细胞间黏附分子-1（sICAM-1）或可溶性血管细胞黏附分子-1（sVCAM-1）没有变化，试验之间也没有差异。白细胞介素-6（IL-6）和白细胞餐后增加（p<0.05）。试验之间IL-6值没有差异。运动试验中0小时白细胞较高，对照试验和运动试验6小时时白细胞值相似。高脂餐会使EMP增加，但sICAM-1或sVCAM-1不会增加。然而，尽管餐后血脂显著降低且HDL-C升高，但急性运动并不会使EMP减少。
