Liu Ling, Li Yan, Liu Shuchun, Zheng Zhihui, Chen Xulin, Zhang Hua, Guo Liangdong, Che Yongsheng
Key Laboratory of Systematic Mycology & Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Org Lett. 2009 Jul 2;11(13):2836-9. doi: 10.1021/ol901039m.
Chloropestolide A (1), a highly functionalized spiroketal with an unprecedented skeleton derived from a chlorinated bicyclo-[2.2.2]-oct-2-en-5-one ring and a 2,6-dihydroxy-4-methylbenzoic acid unit, has been isolated from the scale-up fermentation extract of Pestalotiopsis fici. The structure of 1 was elucidated by NMR spectroscopy and X-ray crystallography. 1 could be derived from the same Diels-Alder precursors as 2 and showed significant inhibitory effects on growth of two human cancer cell lines, HeLa and HT29, with GI(50) values of 0.7 and 4.2 microM, respectively.
氯 Pestolide A（1）是一种高度官能化的螺环缩酮，具有前所未有的骨架，其衍生自氯化双环[2.2.2] -辛-2-烯-5-酮环和2,6-二羟基-4-甲基苯甲酸单元，它是从榕树拟盘多毛孢的放大发酵提取物中分离得到的。1的结构通过核磁共振光谱和X射线晶体学得以阐明。1可能与2来自相同的狄尔斯-阿尔德前体，并且对两种人类癌细胞系HeLa和HT29的生长显示出显著的抑制作用，其GI(50)值分别为0.7和4.2微摩尔。
