Kim Yun-Gon, Moon Sangjun, Kuritzkes Daniel R, Demirci Utkan
Bio-Acoustic MEMS in Medicine Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, United States.
Biosens Bioelectron. 2009 Sep 15;25(1):253-8. doi: 10.1016/j.bios.2009.06.023. Epub 2009 Jun 18.
Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 microl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfluidic chip. Two-color Qdots (Qdot525 and Qdot655 streptavidin conjugates) were used to identify the captured HIV by simultaneous labeling the envelope gp120 glycoprotein and its high-mannose glycans. This dual-stain imaging technique using Qdots provides a new and effective tool for accurate identification of HIV particles from patient whole blood without any pre-processing. This on-chip HIV capture and imaging platform creates new avenues for point-of-care diagnostics and monitoring applications of infectious diseases.
全球范围内，超过3320万人感染了人类免疫缺陷病毒（HIV），这些人大多生活在难以获得适当医疗护理的发展中国家。我们开发了一种芯片上的HIV捕获和成像方法，该方法使用量子点（Qdots），从未经处理的HIV感染患者全血的指尖血量（10微升）中，在固定有抗gp120抗体的微流控芯片上进行操作。使用双色量子点（Qdot525和Qdot655链霉亲和素缀合物），通过同时标记包膜gp120糖蛋白及其高甘露糖聚糖来识别捕获的HIV。这种使用量子点的双染成像技术为无需任何预处理即可从患者全血中准确识别HIV颗粒提供了一种新的有效工具。这种芯片上的HIV捕获和成像平台为即时诊断和传染病监测应用开辟了新途径。
