连续流微流控技术在 HIV 感染研究中的进展。

Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection.

机构信息

Institute of Research in Infectious Diseases (IRIM), Centre National de la Recherche Scientifique (CNRS), UMR9004, University of Montpellier, Team R2D2, 34293 Montpellier, France.

出版信息

Viruses. 2020 Sep 4;12(9):982. doi: 10.3390/v12090982.

DOI:10.3390/v12090982

PMID:32899657

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7552050/

Abstract

HIV-1 is the causative agent of acquired immunodeficiency syndrome (AIDS). It affects millions of people worldwide and the pandemic persists despite the implementation of highly active antiretroviral therapy. A wide spectrum of techniques has been implemented in order to diagnose and monitor AIDS progression over the years. Besides the conventional approaches, microfluidics has provided useful methods for monitoring HIV-1 infection. In this review, we introduce continuous microfluidics as well as the fabrication and handling of microfluidic chips. We provide a review of the different applications of continuous microfluidics in AIDS diagnosis and progression and in the basic study of the HIV-1 life cycle.

摘要

HIV-1 是获得性免疫缺陷综合征（AIDS）的病原体。它影响着全球数百万人，尽管实施了高效抗逆转录病毒疗法，但这种大流行仍在持续。多年来，已经实施了广泛的技术来诊断和监测 AIDS 的进展。除了传统方法之外，微流控技术还为监测 HIV-1 感染提供了有用的方法。在这篇综述中，我们介绍了连续微流控技术以及微流控芯片的制造和处理。我们回顾了连续微流控技术在 AIDS 诊断和进展以及 HIV-1 生命周期基础研究中的不同应用。