Mu Hong-mei, Wang Ren, Li Xiao-dan, Jiang Yu-mei, Wang Chun-yan, Quan Jun-ping, Peng Feng, Xia Bing
College of Life Science, Nanjing Agricultural University, Nanjing, 210095, China.
Z Naturforsch C J Biosci. 2009 Jul-Aug;64(7-8):541-50. doi: 10.1515/znc-2009-7-813.
Three-month-old seedlings of Lycoris chinensis were treated with biotic and abiotic elicitors: yeast elicitor (YE), methyl jasmonate (MJ), salicylic acid (SA), and sodium nitroprusside as NO donator (NO). We have shown that the addition of MJ and NO promotes the accumulation of galanthamine in these seedlings. The effect of these elicitors on the growth of the seedlings, as well as on the amount of the alkaloids accumulated in the seedlings was studied. The results showed that, in general, high doses of MJ and SA had a negative effect on the growth of the seedlings, while appropriate doses of NO and YE had a positive effect on the growth of the seedlings. It was remarkable that the addition of MJ, NO, and YE can promote galanthamine accumulation in seedlings. The accumulation was higher in treatments at higher concentrations of NO (100 microM), where the release of galanthamine was 1.72-fold higher than that of the control at the 10th day of culture. The highest values of lycorine were obtained in seedlings treated with YE at a concentration of 0.01 g/l and by the 10th day of culture; the level was 1.38 times of the control.
酵母诱导剂（YE）、茉莉酸甲酯（MJ）、水杨酸（SA）以及作为一氧化氮供体的硝普钠（NO）。我们已经表明，添加MJ和NO可促进这些幼苗中加兰他敏的积累。研究了这些诱导剂对幼苗生长以及幼苗中生物碱积累量的影响。结果表明，总体而言，高剂量的MJ和SA对幼苗生长有负面影响，而适当剂量的NO和YE对幼苗生长有正面影响。值得注意的是，添加MJ、NO和YE可促进幼苗中加兰他敏的积累。在较高浓度NO（100微摩尔）处理中积累量更高，在培养第10天时加兰他敏的释放量比对照高1.72倍。在用浓度为0.01克/升的YE处理的幼苗中，到培养第10天时获得了最高的石蒜碱值；该水平是对照的1.38倍。
