Escuela Nacional de Ciencias Biológicas, Laboratorio de Toxicología Acuática, Departamento de Farmacia, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Col. Industrial Vallejo, Mexico, DF, Mexico.
Fish Physiol Biochem. 2010 Dec;36(4):875-82. doi: 10.1007/s10695-009-9363-1. Epub 2009 Oct 29.
Several studies of fish have shown that aluminum may induce hypoxia, hypercapnia, metabolic acidosis, and respiratory failure. In lymphocytes, morphologic abnormalities and reduced immune activity have been observed. Nevertheless, there is little data on oxidative stress and such data are essential in order to identify its mechanism of action. The common carp Cyprinus carpio, an omnivorous fish commonly used in commercial aquaculture, has been proposed as a test organism in toxicologic assays due to its economic importance and wide geographic distribution. The aim of this work was to evaluate Al-induced oxidative stress in lymphocytes of the common carp C. carpio. Specimens were exposed to three different concentrations of Al (0.05, 120, and 239 mg/l) in a static exposure system for 96 h. At the end of the exposure period, blood was collected and lymphocytes were separated. Lipid peroxidation, oxidized protein content and the activity of superoxide dismutase, catalase, and glutathione peroxidase were measured. Results show that the tested Al concentrations modified the activity of antioxidant enzymes and elicited higher levels of lipid peroxidation and oxidized proteins. The degree of damage induced was concentration and tissue dependent.
几项鱼类研究表明，铝可能会诱发缺氧、高碳酸血症、代谢性酸中毒和呼吸衰竭。在淋巴细胞中，已经观察到形态异常和免疫活性降低。然而，关于氧化应激的数据很少，这些数据对于确定其作用机制至关重要。鲤鱼是一种杂食性鱼类，常用于商业水产养殖，由于其经济重要性和广泛的地理分布，已被提议作为毒理学检测中的试验生物。本工作旨在评估鲤鱼淋巴细胞中铝诱导的氧化应激。标本在静态暴露系统中暴露于三种不同浓度的铝（0.05、120 和 239mg/l）96 小时。暴露期结束时，采集血液并分离淋巴细胞。测量脂质过氧化、氧化蛋白含量以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。结果表明，测试的铝浓度改变了抗氧化酶的活性，并引起更高水平的脂质过氧化和氧化蛋白。诱导的损伤程度与浓度和组织有关。
