Binnicker M J, Jespersen D J, Harring J A
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Clin Vaccine Immunol. 2010 Feb;17(2):253-7. doi: 10.1128/CVI.00325-09. Epub 2009 Dec 9.
The diagnosis of herpes simplex virus (HSV) infections is routinely made based on clinical findings and supported by laboratory testing using PCR or viral culture. However, in instances of subclinical or unrecognized HSV infection, serologic testing for IgG class antibodies to type-specific HSV glycoprotein G (gG) may be useful. This study evaluated and compared the performances of three multiplex flow immunoassays (AtheNA Multi-Lyte [Zeus Scientific], BioPlex 2200 [Bio-Rad Laboratories], and Plexus HerpeSelect [Focus Diagnostics]) for the simultaneous detection of gG type-specific IgG antibodies to HSV types 1 and 2 (HSV-1 and HSV-2). Serum specimens (n = 505) submitted for routine gG type-specific HSV IgG testing by enzyme immunoassay (EIA) (HerpeSelect; Focus Diagnostics) were also tested by the three multiplex flow immunoassays. Specimens showing discordant results were tested by HSV type-specific Western blotting (WB). For HSV-1 IgG, the AtheNA, BioPlex, and Plexus assays demonstrated agreements of 94.9% (479/505 specimens), 97.8% (494/505 specimens), and 97.4% (492/505 specimens), respectively, with the results of EIA. For HSV-2 IgG, the AtheNA, BioPlex, and Plexus assays showed agreements of 87.9% (444/505 specimens), 97.2% (491/505 specimens), and 96.8% (489/505 specimens), respectively, with EIA results. Timing studies showed that the AtheNA, BioPlex, and Plexus assays could provide complete analysis of 90 serum specimens in 3.1, 1.5, and 2.9 h, respectively, versus 3.1 h by EIA. These findings suggest that the gG type-specific HSV IgG multiplex immunoassays may be beneficial to high-volume clinical laboratories experiencing significant increases in the number of specimens submitted for HSV serologic testing. The evaluated systems provide comparable results to those of EIA, while reducing hands-on time and eliminating the necessity to aliquot specimens prior to testing.
单纯疱疹病毒（HSV）感染的诊断通常基于临床表现，并通过使用聚合酶链反应（PCR）或病毒培养的实验室检测来支持。然而，在亚临床或未被识别的HSV感染情况下，针对特定型别的HSV糖蛋白G（gG）的IgG类抗体的血清学检测可能会有所帮助。本研究评估并比较了三种多重流式免疫分析方法（AtheNA Multi-Lyte [宙斯科学公司]、BioPlex 2200 [伯乐公司]和Plexus HerpeSelect [焦点诊断公司]）同时检测HSV-1和HSV-2型特异性gG IgG抗体的性能。通过酶免疫分析（EIA）（HerpeSelect；焦点诊断公司）进行常规gG型特异性HSV IgG检测提交的血清标本（n = 505）也采用这三种多重流式免疫分析方法进行检测。对结果不一致的标本采用HSV型特异性免疫印迹法（WB）进行检测。对于HSV-1 IgG，AtheNA、BioPlex和Plexus分析方法与EIA结果的一致性分别为94.9%（479/505个标本）、97.8%（494/505个标本）和97.4%（492/505个标本）。对于HSV-2 IgG，AtheNA、BioPlex和Plexus分析方法与EIA结果的一致性分别为87.9%（444/505个标本）、97.2%（491/505个标本）和96.8%（489/505个标本）。时间研究表明，AtheNA、BioPlex和Plexus分析方法分别可在3.1小时、1.5小时和2.9小时内对90份血清标本进行完整分析，而EIA则需要3.1小时。这些发现表明，gG型特异性HSV IgG多重免疫分析方法可能对提交HSV血清学检测标本数量大幅增加的高容量临床实验室有益。所评估的系统提供了与EIA相当的结果，同时减少了实际操作时间，并消除了检测前对标本进行分装的必要性。
