一种来自昆虫病原真菌球孢白僵菌 BCC 16475 的抗分枝杆菌环二肽。

An antimycobacterial cyclodepsipeptide from the entomopathogenic fungus Ophiocordyceps communis BCC 16475.

机构信息

National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Klong Luang, Pathumthani 12120, Thailand.

出版信息

J Nat Prod. 2010 Jan;73(1):75-8. doi: 10.1021/np900520b.

DOI:10.1021/np900520b

PMID:20028029

Abstract

A novel cyclodepsipeptide, cordycommunin (1), and two dihydroisocoumarins (2 and 3) were isolated from the insect pathogenic fungus Ophiocordyceps communis BCC 16475. The absolute configurations of the amino acid residues of 1 were addressed by application of Marfey's method. Cordycommunin (1) showed growth inhibition of Mycobacterium tuberculosis H37Ra with an MIC value of 15 microM. This compound also exhibited weak cytotoxicity to KB cells with an IC50 of 45 microM, while it was inactive against BC, NCI-H187, and Vero cell lines at a concentration of 88 microM (50 microg/mL).

摘要

从昆虫病原真菌蛹草拟青霉 BCC 16475 中分离得到一种新型环二肽化合物 cordycommunin（1）和两种二氢异香豆素（2 和 3）。采用 Marfey 法确定了 1 的氨基酸残基的绝对构型。Cordycommunin（1）对结核分枝杆菌 H37Ra 的生长具有抑制作用，MIC 值为 15 μM。该化合物对 KB 细胞也表现出较弱的细胞毒性，IC50 为 45 μM，而在 88 μM（50 μg/mL）浓度下对 BC、NCI-H187 和 Vero 细胞系无活性。