Division of Biomolecular Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
J Control Release. 2010 Apr 19;143(2):258-64. doi: 10.1016/j.jconrel.2009.12.029. Epub 2010 Jan 13.
To develop a drug carrier that enables time-programmed dual release in a single formulation, multilayered drug-loaded biodegradable nanofiber meshes were designed using sequential electrospinning with the following construction: (i) first drug-loaded mesh (top), (ii) barrier mesh, (iii) second drug-loaded mesh, and (iv) basement mesh (bottom). The drug release speed and duration were controlled by designing morphological features of the electrospun meshes such as the fiber diameter and mesh thickness. Control of the timed release of the second drug-the retardation period-was accomplished by appropriate design of the barrier mesh thickness. An in vitro release experiment demonstrated that the tetra-layered construction described above with appropriate morphological features of each component mesh can provide timed dual release of the respective drugs. The time-programmed dual release system using the multilayered electrospun nanofiber meshes was demonstrated as a useful formulation for advanced multidrug combination therapy requiring regiospecific administration of different drugs at different times. The potential use of the present multilayered formulation is discussed for application to biochemical modulation as one administrative strategy for use in sequential chemotherapy employing multiple anti-tumor drugs.
为了开发一种能够在单一配方中实现时间控制的双重释放的药物载体，我们使用顺序静电纺丝设计了多层载药可生物降解纳米纤维网，其结构如下：（i）第一载药网（顶部），（ii）阻隔网，（iii）第二载药网，和（iv）基底网（底部）。通过设计电纺网的形态特征，如纤维直径和网厚，可以控制药物的释放速度和持续时间。通过适当设计阻隔网的厚度来控制第二药物的定时释放-延迟期。体外释放实验表明，上述具有各组件网适当形态特征的四层结构可以提供各自药物的定时双重释放。使用多层电纺纳米纤维网的时间控制双重释放系统被证明是一种有用的制剂，适用于需要在不同时间对不同部位给予不同药物的先进多药联合治疗。讨论了本多层制剂在生化调节中的潜在用途，作为一种用于使用多种抗肿瘤药物进行序贯化疗的行政策略。
