Gene Regulation Programme, Centre de Regulació Genòmica (CRG-UPF), 08003 Barcelona, Spain.
Genes Dev. 2010 Jan 15;24(2):129-34. doi: 10.1101/gad.568610.
Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation that requires two sequences in the 3' untranslated region (UTR) of vertebrate substrates: the polyadenylation hexanucleotide, and the cytoplasmic polyadenylation element (CPE). Using a cell-free Drosophila system, we show that these signals are not relevant for Toll polyadenylation but, instead, a "polyadenylation region" (PR) is necessary. Competition experiments indicate that PR-mediated polyadenylation is required for viability and is mechanistically distinct from the CPE/hexanucleotide-mediated process. These data indicate that Toll mRNA is polyadenylated by a noncanonical mechanism, and suggest that a novel machinery functions for cytoplasmic polyadenylation during Drosophila embryogenesis.
细胞质多聚腺苷酸化是一种广泛存在的调节 mRNA 翻译的机制，它需要脊椎动物底物 3'非翻译区（UTR）中的两个序列：多聚腺苷酸化六核苷酸和细胞质多聚腺苷酸化元件（CPE）。使用无细胞的果蝇系统，我们表明这些信号与 Toll 的多聚腺苷酸化无关，而是需要一个“多聚腺苷酸化区”（PR）。竞争实验表明，PR 介导的多聚腺苷酸化对于生存是必需的，并且在机制上与 CPE/六核苷酸介导的过程不同。这些数据表明 Toll mRNA 是通过一种非典型的机制进行多聚腺苷酸化的，并表明在果蝇胚胎发生过程中，一种新的机制用于细胞质多聚腺苷酸化。
