Key Laboratory for Oral Biomedical Engineering, Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, PR China.
J Dent. 2010 May;38(5):400-10. doi: 10.1016/j.jdent.2010.01.007. Epub 2010 Jan 25.
The purpose of this study was to evaluate the in vitro effectiveness of a new bioglass-containing and two commercial desensitising toothpastes on dentinal tubule occlusion after citric acid challenge or artificial saliva (AS) immersion.
One hundred dentin discs from human third molars were used. Specimens were randomly divided into five groups (n=20), Group 1: EDTA-treated dentin; Group 2: brushing with distilled water; Group 3: brushing with Novamin; Group 4: brushing with Sensodyne Freshmint; Group 5: brushing with Colgate Sensitive. In each group, samples were then equally split into two subgroups (n=10) to test two post-treatments: 6% citric acid challenge or 24h immersion in artificial saliva. Dentine permeability of each specimen was measured before and after each treatment using a hydrostatic device working at 20cm H(2)O pressure. Data were analysed by two-way repeated measures ANOVA to determine if there were any significant differences within or between groups. Dentine morphology and surface deposits were observed by SEM.
All three desensitising toothpastes significantly reduced dentine permeability and created precipitates on the treated dentine surfaces. Moreover, the reductions in dentine permeability showed partial recovery after a citric acid and artificial saliva immersion. Sensodyne showed significant resistant to acid attack and Novamin exhibited the lowest permeability after artificial saliva immersion for 24h.
The application of the three toothpastes resulted in effective dentinal tubule occlusion. However, the new bioglass-containing toothpaste (Novamin) represented excellent occlusion effects after brushing treatment and AS immersion, while Sensodyne demonstrated more reduction in permeability when citric acid challenged.
本研究旨在评估一种新型含生物玻璃的脱敏牙膏和两种市售脱敏牙膏在柠檬酸挑战或人工唾液（AS）浸泡后对牙本质小管封闭的体外效果。
使用 100 个人第三磨牙的牙本质圆盘。标本随机分为五组（n=20），组 1：EDTA 处理牙本质；组 2：用蒸馏水刷牙；组 3：用 Novamin 刷牙；组 4：用 Sensodyne Freshmint 刷牙；组 5：用 Colgate Sensitive 刷牙。在每组中，样本又均等分为两个亚组（n=10），以测试两种后处理：6%柠檬酸挑战或 24 小时人工唾液浸泡。使用在 20cm H2O 压力下工作的静水设备测量每个标本在每次处理前后的牙本质通透性。数据采用双向重复测量方差分析进行分析，以确定组内或组间是否存在任何显著差异。通过 SEM 观察牙本质形态和表面沉积物。
三种脱敏牙膏均显著降低牙本质通透性，并在处理后的牙本质表面形成沉淀物。此外，柠檬酸和人工唾液浸泡后，牙本质通透性的降低显示出部分恢复。Sensodyne 对酸攻击具有显著的抵抗力，Novamin 在人工唾液浸泡 24 小时后表现出最低的通透性。
三种牙膏的应用均导致牙本质小管有效封闭。然而，新型含生物玻璃的牙膏（Novamin）在刷牙处理和 AS 浸泡后表现出出色的封闭效果，而 Sensodyne 在柠檬酸挑战时表现出更低的通透性。
