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Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores.
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Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly.
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Phosphorylation by Cdk1 increases the binding of Eg5 to microtubules in vitro and in Xenopus egg extract spindles.
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The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations.
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Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules.
Cell. 2008 Nov 28;135(5):894-906. doi: 10.1016/j.cell.2008.09.046.
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Dynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly.
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Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles.
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Dynamic partitioning of mitotic kinesin-5 cross-linkers between microtubule-bound and freely diffusing states.
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Microtubule cross-linking triggers the directional motility of kinesin-5.
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