Van Zijl P C, Moonen C T, Faustino P, Pekar J, Kaplan O, Cohen J S
National Cancer Institute, Medicine Branch, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3228-32. doi: 10.1073/pnas.88.8.3228.
A method is outlined that completely separates intracellular and extracellular information in NMR spectra of perfused cells. The technique uses diffusion weighting to exploit differences in motional properties between intra- and extracellular constituents. This allows monitoring of intracellular metabolism, and of transport of small drugs and nutrients through the cell membrane, under controlled physiological conditions. As a first example, proton spectra of drug-resistant MCF-7 human breast cancer cells are studied, and uptake of phenylalanine is monitored.
本文概述了一种在灌注细胞的核磁共振波谱中完全分离细胞内和细胞外信息的方法。该技术利用扩散加权来利用细胞内和细胞外成分在运动特性上的差异。这使得在可控的生理条件下能够监测细胞内代谢以及小分子药物和营养物质通过细胞膜的转运。作为第一个例子，研究了耐药性MCF - 7人乳腺癌细胞的质子谱，并监测了苯丙氨酸的摄取情况。
