Facultad de Ciencias Exactas, Laboratorio de Investigaciones del Sistema Inmune (LISIN), UNLP 47 y 115, 1900, La Plata, Argentina.
Med Microbiol Immunol. 2010 May;199(2):103-8. doi: 10.1007/s00430-010-0142-5. Epub 2010 Feb 9.
Non-specific enhancement of the airways innate response has been shown to impair lung infections in several models of infection such diverse as influenza A, Streptococcus pneumoniae, and Aspergillus niger. Our aim was to evaluate whether a similar event could operate in the context of Bordetella pertussis respiratory infection, not only to enrich the knowledge of host-bacteria interaction but also to establish immunological basis for the development of new control strategies against the pathogen. Using a B. pertussis intranasal infection model and coadministration of different TLR agonists at the moment of the infection, we observed that the enhancement of innate response activation, in a TLR4-dependent way, could efficiently impair B. pertussis colonization (P < 0.001). While LPS from different microbial sources were equally effective in promoting this effect, flagellin and poly I:C coadministration, in spite of inducing expression of innate response markers TNFalpha, CXCL2, CXCL10 and IL6, was not effective to prevent B. pertussis colonization. Our results indicate that during the early stage of infection, specific anti-microbial mechanisms triggered by TLR4 stimulation are able to impair B. pertussis colonization. These findings could complement our current view of the role of TLR4-dependent processes that contribute to anti-pertussis immunity.
非特异性增强气道先天反应已被证明可损害多种感染模型中的肺部感染，如流感 A、肺炎链球菌和黑曲霉。我们的目的是评估在百日咳博德特氏菌呼吸道感染的背景下是否会发生类似的事件，这不仅丰富了宿主-细菌相互作用的知识，而且为开发针对病原体的新控制策略奠定了免疫学基础。使用百日咳博德特氏菌鼻内感染模型和在感染时同时给予不同 TLR 激动剂，我们观察到先天反应激活的增强（以 TLR4 依赖性方式）可有效抑制百日咳博德特氏菌定植（P < 0.001）。虽然来自不同微生物来源的 LPS 在促进这种作用方面同样有效，但鞭毛蛋白和聚 I:C 共同给药虽然诱导了先天反应标志物 TNFalpha、CXCL2、CXCL10 和 IL6 的表达，但不能有效预防百日咳博德特氏菌定植。我们的结果表明，在感染的早期阶段，TLR4 刺激引发的特定抗微生物机制能够抑制百日咳博德特氏菌的定植。这些发现可以补充我们目前对 TLR4 依赖性过程在抗百日咳免疫中的作用的认识。
