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互补的遗传和基因组方法有助于描述大豆第 I 连锁群种子蛋白 QTL。

Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean.

机构信息

United States Department of Agriculture-Agricultural Research Service, Plant Research Unit, St Paul, MN 55108, USA.

出版信息

BMC Plant Biol. 2010 Mar 3;10:41. doi: 10.1186/1471-2229-10-41.

DOI:10.1186/1471-2229-10-41
PMID:20199683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2848761/
Abstract

BACKGROUND

The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content.

RESULTS

A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix Soy GeneChip and high-throughput Illumina whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region.

CONCLUSIONS

This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.

摘要

背景

许多作物的营养价值和经济价值在很大程度上取决于种子蛋白质和油分的含量。为了指导作物改良，需要深入了解这些成分在发育中的种子中沉积的遗传和分子控制机制。大豆（Glycine max (L.) Merrill）连锁群 I（LG I）上的一个数量性状位点（QTL）对种子蛋白质含量有显著影响。

结果

利用大豆近等基因系（NIL）对，在种子蛋白质含量上存在差异，并且在包含 LG I 蛋白质 QTL 的导入基因组片段上存在差异，作为资源来划定 QTL 区域，并研究发育中的种子中转录本丰度的变化。LG I QTL 区域被划定为染色体 20 上小于 8.4 Mbp 的基因组序列。使用 Affymetrix Soy GeneChip 和高通量 Illumina 全转录组测序平台，在 NIL 之间鉴定出了 13 个显示出显著种子转录积累差异的基因，这些基因映射到 8.4 Mbp 的 LG I 蛋白质 QTL 区域。

结论

本研究鉴定了 LG I 蛋白质 QTL 上的候选基因，这些基因可能参与调节大豆种子中的蛋白质含量。研究结果证明了互补方法的强大功能，可用于描述具有对比性的 NIL，并提供了对种子生物学和大豆基因组的全基因组转录组的深入了解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/058df10e55c7/1471-2229-10-41-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/101ca242d78a/1471-2229-10-41-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/b42335cb4bb6/1471-2229-10-41-2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/3d5c77531a4b/1471-2229-10-41-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/7a9a3e136e65/1471-2229-10-41-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/058df10e55c7/1471-2229-10-41-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/101ca242d78a/1471-2229-10-41-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/b42335cb4bb6/1471-2229-10-41-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/6a76d2adc079/1471-2229-10-41-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/d59d21e6d3bc/1471-2229-10-41-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/3d5c77531a4b/1471-2229-10-41-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/7a9a3e136e65/1471-2229-10-41-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81b6/2848761/058df10e55c7/1471-2229-10-41-7.jpg

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本文引用的文献

1
Expression of storage-protein genes during soybean seed development.大豆种子发育过程中贮藏蛋白基因的表达。
Planta. 1981 Oct;153(2):130-9. doi: 10.1007/BF00384094.
2
RFLP analysis of soybean seed protein and oil content.大豆种子蛋白和油含量的 RFLP 分析。
Theor Appl Genet. 1992 Mar;83(5):608-12. doi: 10.1007/BF00226905.
3
Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.).大豆（Glycine max L.）繁殖、形态和种子性状数量性状基因座的区间作图。
丹白孔高蛋白等位基因在不同大豆遗传背景中的渐渗。
Front Plant Sci. 2023 Dec 20;14:1308731. doi: 10.3389/fpls.2023.1308731. eCollection 2023.
4
QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean.大豆两个重组自交系群体种子蛋白质含量的QTLs及候选基因
Plants (Basel). 2023 Oct 16;12(20):3589. doi: 10.3390/plants12203589.
5
Genetic mapping and functional genomics of soybean seed protein.大豆种子蛋白的遗传图谱构建与功能基因组学研究
Mol Breed. 2023 Apr 12;43(4):29. doi: 10.1007/s11032-023-01373-5. eCollection 2023 Apr.
6
Quantitative Trait Loci (QTL) Analysis of Seed Protein and Oil Content in Wild Soybean ().野生大豆种子蛋白和油含量的数量性状位点(QTL)分析。
Int J Mol Sci. 2023 Feb 17;24(4):4077. doi: 10.3390/ijms24044077.
7
Soybean Protein and Oil Variants Identified through a Forward Genetic Screen for Seed Composition.通过正向遗传学筛选种子成分鉴定出的大豆蛋白和油变体
Plants (Basel). 2022 Nov 3;11(21):2966. doi: 10.3390/plants11212966.
8
Soybean genetic resources contributing to sustainable protein production.大豆遗传资源对可持续蛋白质生产的贡献。
Theor Appl Genet. 2022 Nov;135(11):4095-4121. doi: 10.1007/s00122-022-04222-9. Epub 2022 Oct 14.
9
Ensuring Global Food Security by Improving Protein Content in Major Grain Legumes Using Breeding and 'Omics' Tools.利用育种和“组学”工具提高主要粮食豆类作物的蛋白质含量，确保全球粮食安全。
Int J Mol Sci. 2022 Jul 12;23(14):7710. doi: 10.3390/ijms23147710.
10
Haplotype mapping uncovers unexplored variation in wild and domesticated soybean at the major protein locus cqProt-003.单倍型图谱揭示了主要蛋白基因座 cqProt-003 中野生和栽培大豆的未探索变异。
Theor Appl Genet. 2022 Apr;135(4):1443-1455. doi: 10.1007/s00122-022-04045-8. Epub 2022 Feb 9.
Theor Appl Genet. 1993 Sep;86(8):907-13. doi: 10.1007/BF00211040.
4
RFLP loci associated with soybean seed protein and oil content across populations and locations.与大豆种子蛋白和油含量相关的 RFLP 位点在不同群体和地点的分布情况。
Theor Appl Genet. 1996 Oct;93(5-6):649-57. doi: 10.1007/BF00224058.
5
Genome sequence of the palaeopolyploid soybean.古多倍体大豆基因组序列。
Nature. 2010 Jan 14;463(7278):178-83. doi: 10.1038/nature08670.
6
Single-feature polymorphism discovery by computing probe affinity shape powers.通过计算探针亲和力形状幂来发现单特征多态性。
BMC Genet. 2009 Aug 26;10:48. doi: 10.1186/1471-2156-10-48.
7
A transcriptomic analysis of superhybrid rice LYP9 and its parents.超级杂交水稻LYP9及其亲本的转录组分析
Proc Natl Acad Sci U S A. 2009 May 12;106(19):7695-701. doi: 10.1073/pnas.0902340106. Epub 2009 Apr 16.
8
The proteome of seed development in the model legume Lotus japonicus.模式豆科植物百脉根种子发育的蛋白质组
Plant Physiol. 2009 Mar;149(3):1325-40. doi: 10.1104/pp.108.133405. Epub 2009 Jan 7.
9
The role of light in soybean seed filling metabolism.光在大豆种子灌浆代谢中的作用。
Plant J. 2009 Apr;58(2):220-34. doi: 10.1111/j.1365-313X.2008.03771.x. Epub 2008 Dec 10.
10
Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses.参与拟南芥分化和应激反应的新型长链非蛋白质编码RNA
Genome Res. 2009 Jan;19(1):57-69. doi: 10.1101/gr.080275.108. Epub 2008 Nov 7.