Zaia J A, Leary P L, Levin M J
Infect Immun. 1978 Jun;20(3):646-51. doi: 10.1128/iai.20.3.646-651.1978.
Peripheral blood mononuclear (PBM) cells from donors with a history of prior infection with herpes simplex virus, varicella-zoster virus, and/or cytomegalovirus were cultured for 2 to 8 days with glycine-extracted antigens prepared from these viruses and from infectious bovine rhinotracheitis virus. The proliferative response of PBM cells from all donors was specific during the first 6 days in culture. During this period the cellular immune responses of the seronegative donors were clearly different from those of the seropositive donors. The responses of PBM cells in culture with any of the human herpesvirus antigens studied was not influenced by prior infection of the donor with one or more other human herpesviruses. In contrast, although no donors had antibody to infectious bovine rhinotracheitis virus, the PBM cells from some of them had a proliferative response to this bovine herpesvirus, which increased with time. This nonspecific response appears to be a host-associated function which may be related to recognition of a common herpesvirus antigen.
从有单纯疱疹病毒、水痘-带状疱疹病毒和/或巨细胞病毒既往感染史的供体采集的外周血单核（PBM）细胞，与用这些病毒以及传染性牛鼻气管炎病毒制备的甘氨酸提取抗原一起培养2至8天。在培养的前6天，所有供体的PBM细胞的增殖反应都是特异性的。在此期间，血清阴性供体的细胞免疫反应与血清阳性供体的明显不同。用所研究的任何一种人类疱疹病毒抗原培养时，PBM细胞的反应不受供体先前感染一种或多种其他人类疱疹病毒的影响。相比之下，尽管没有供体具有针对传染性牛鼻气管炎病毒的抗体，但其中一些供体的PBM细胞对这种牛疱疹病毒有增殖反应，且该反应随时间增加。这种非特异性反应似乎是一种宿主相关功能，可能与对共同疱疹病毒抗原的识别有关。
