Graduate Institute of Biotechnology, Chinese Culture University, 55 Hwa-Kong Road, Taipei, 111, Taiwan, ROC.
Plant Cell Rep. 2010 Jun;29(6):575-93. doi: 10.1007/s00299-010-0846-4. Epub 2010 Mar 30.
The objectives of this work were to clone the catalase (CAT) gene from broccoli (Brassica oleracea) and the ascorbate peroxidase (APX) gene from Chinese cabbage and measure the regulation of CAT and APX gene expressions under heat-stress conditions. Different genotypes responded differently to heat stress according to their various antioxidant enzymes and physiological parameters. CAT and APX gene expression profiles were well matched with the data for CAT and APX enzyme activities in the broccoli and Chinese cabbage plants, respectively. Full-length of the CAT and APX cDNA were 1,768 and 1,070 bp, respectively. A phylogenetic analysis of CAT and APX indicated that plant CATs and APXs diverged into two major clusters.
本研究旨在从西兰花（Brassica oleracea）中克隆过氧化氢酶（CAT）基因，从白菜中克隆抗坏血酸过氧化物酶（APX）基因，并测定 CAT 和 APX 基因在热胁迫条件下的表达调控。不同基因型的植物根据其不同的抗氧化酶和生理参数对热胁迫的反应不同。CAT 和 APX 基因的表达谱与西兰花和白菜植物中 CAT 和 APX 酶活性的数据非常匹配。CAT 和 APX 的全长 cDNA 分别为 1768 和 1070 bp。CAT 和 APX 的系统发育分析表明，植物 CATs 和 APXs 分为两个主要聚类。
