Siqueira M M, Nascimento J P, Anderson L J
Departmento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
J Clin Microbiol. 1991 Mar;29(3):557-9. doi: 10.1128/jcm.29.3.557-559.1991.
The antigenic characteristics of 87 strains of respiratory syncytial virus isolated in Rio de Janeiro, Brazil, from 1982 to 1988 were determined with a panel of monoclonal antibodies (MAbs) in an enzyme immunoassay. Four of these MAbs immunoprecipitated the fusion protein, and five immunoprecipitated the large glycoprotein. On the basis of the patterns of reaction of these MAbs to respiratory syncytial virus isolates in an enzyme immunoassay, we were able to separate isolates into the two major groups, A and B, and also to identify four variants within group A and three within group B. Strains from groups A and B were isolated each year, and the prevalence of the two groups varied over the seven study years.
1982年至1988年期间，在巴西里约热内卢分离出87株呼吸道合胞病毒，利用一组单克隆抗体（MAb）通过酶免疫测定法确定了这些病毒的抗原特性。其中4种MAb免疫沉淀融合蛋白，5种免疫沉淀大糖蛋白。根据这些MAb在酶免疫测定中与呼吸道合胞病毒分离株的反应模式，我们能够将分离株分为A和B两大组，还能在A组中鉴定出4个变体，在B组中鉴定出3个变体。每年都分离出A组和B组的毒株，在7年的研究期间，两组的流行率有所不同。
