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从墨西哥尤卡坦半岛的白纹伊蚊和致倦库蚊中分离出黄病毒并进行序列分析。

Isolation and sequence analysis of Culex flavivirus from Culex interrogator and Culex quinquefasciatus in the Yucatan Peninsula of Mexico.

机构信息

Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.

出版信息

Arch Virol. 2010 Jun;155(6):983-6. doi: 10.1007/s00705-010-0665-5. Epub 2010 Apr 9.

DOI:10.1007/s00705-010-0665-5
PMID:20379749
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2933740/
Abstract

Previously, we reported a high prevalence of Culex flavivirus (CxFV) in Culex quinquefasciatus (Say) in the Yucatan Peninsula of Mexico. To determine whether other Culex spp. mosquitoes in this region are susceptible to natural CxFV infection, Cx. bahamensis (Dyar and Knab), Cx. coronator (Dyar and Knab), Cx. interrogator (Dyar and Knab), Cx. nigripalpus (Theobald) and Cx. opisthopus (Komp) in the Yucatan Peninsula of Mexico were tested for CxFV. Two pools of Cx. interrogator were positive. The envelope protein genes of these isolates and 16 isolates from Cx. quinquefasciatus were sequenced and shown to have > or =99.2% nucleotide identity. These data suggest that there is limited genetic diversity among CxFV isolates in the Yucatan Peninsula of Mexico.

摘要

先前,我们报道了在墨西哥尤卡坦半岛的致倦库蚊(Culex quinquefasciatus (Say))中存在高流行的黄病毒属库蚊病毒(Culex flavivirus,CxFV)。为了确定该地区的其他库蚊属蚊子是否容易受到自然 CxFV 感染,我们对来自墨西哥尤卡坦半岛的库蚊属埃及伊蚊(Culex bahamensis (Dyar and Knab))、库蚊属库蚊(Culex coronator (Dyar and Knab))、库蚊属曼蚊(Culex interrogator (Dyar and Knab))、库蚊属黑带库蚊(Culex nigripalpus (Theobald))和库蚊属库蚊(Culex opisthopus (Komp))进行了 CxFV 检测。两个库蚊属曼蚊池呈阳性。这些分离株的包膜蛋白基因和来自致倦库蚊的 16 个分离株进行了测序,并显示出>或=99.2%的核苷酸同一性。这些数据表明,墨西哥尤卡坦半岛的 CxFV 分离株之间存在有限的遗传多样性。

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