Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, 66421, Homburg, Saar, Germany.
Physical Chemistry and Didactics of Chemistry, Saarland University, Campus B2 2, 66123, Saarbrücken, Germany.
Clin Oral Investig. 2021 Nov;25(11):6109-6118. doi: 10.1007/s00784-021-03910-y. Epub 2021 Apr 6.
This in situ study aimed to determine and compare the chlorhexidine (CHX) retention in the oral cavity after the application of different CHX pharmaceutical regimens.
Five volunteers used different CHX treatment regimens including mouth rinses, dental spray and toothpaste gel. After the application of the different CHX regimens, 2-μl samples were taken from saliva and buccal mucosa pellicle as well as the dental pellicle samples formed on standardized enamel surfaces. Sample collection was conducted at six time points within 12 h. Retention of CHX was measured using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
CHX retention values in the oral mucosa pellicle were significantly higher than those in saliva. CHX remained in the mucosal pellicle at microgrammes per millilitre levels for 12 h after mouth rinsing, 10 h after spray application and 2 h after using the toothpaste. CHX was detected in the dental pellicle for at least 12 h after application of mouth rinsing and spray. Retention of CHX after mouth rinsing or spray application was significantly higher than the retention after using toothpaste.
Oral mucosa was the favourable site for CHX retention. Higher mouth rinse concentration and longer rinsing time produced a slight increase in CHX retention. CHX spray provided considerable retention values, whereas toothpaste gel delivered the lowest retention after application. MALDI-TOF was a sensitive method with excellent limits of quantification for CHX detection.
本原位研究旨在确定和比较不同洗必泰（CHX）给药方案在口腔中的保留情况。
5 名志愿者使用不同的 CHX 治疗方案，包括漱口、口腔喷雾和牙膏凝胶。在使用不同的 CHX 方案后，在 12 小时内的 6 个时间点，从唾液和颊黏膜上皮以及标准化牙釉质表面形成的牙菌斑样本中采集 2μl 样本。采用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法测定 CHX 的保留情况。
口腔黏膜上皮中的 CHX 保留值明显高于唾液中的保留值。漱口后 12 小时、喷雾后 10 小时和使用牙膏后 2 小时，CHX 仍以微克/毫升的水平保留在黏膜上皮中。漱口和喷雾后，CHX 至少在 12 小时内可检测到牙菌斑。漱口或喷雾后 CHX 的保留明显高于使用牙膏后 CHX 的保留。
口腔黏膜是 CHX 保留的有利部位。较高的漱口液浓度和较长的漱口时间可略微增加 CHX 的保留。CHX 喷雾提供了相当高的保留值，而牙膏凝胶在使用后提供的保留值最低。MALDI-TOF 是一种灵敏的方法，对 CHX 的检测具有优异的定量限。
