Bielak Lawrence F, Horenstein Richard B, Ryan Kathleen A, Sheedy Patrick F, Rumberger John A, Tanner Keith, Post Wendy, Mitchell Braxton D, Shuldiner Alan R, Peyser Patricia A
Department of Epidemiology, University of Michigan, Ann Arbor, Michigan.
Clin Med Cardiol. 2009 May 27;3:53-60. doi: 10.4137/cmc.s2111.
Bone-marrow derived progenitor cells (PCs) may play a role in maintaining vascular health by actively repairing damaged endothelium. The purpose of this study in asymptomatic Old Order Amish men (n = 90) without hypertension or diabetes was to determine if PC count, as determined by CD34+ cell count in peripheral blood, was associated with 10-year risk of cardiovascular disease (CVD) and measures of subclinical atherosclerosis.
CD34+ cell count by fluorescence-activated cell sorting, coronary artery calcification (CAC) by electron beam computed tomography, and CVD risk factors were obtained. Carotid intimal-medial thickness (CIMT) also was obtained in a subset of 57 men. After adjusting for 10-year CVD risk, CD34+ cell count was significantly associated with CAC quantity (p = 0.03) and CIMT (p < 0.0001). A 1-unit increase in natural-log transformed CD34+ cell count was associated with an estimated 55.2% decrease (95% CI: -77.8% to -9.3%) in CAC quantity and an estimated 14.3% decrease (95% CI: -20.1% to -8.1%) in CIMT.
Increased CD34+ cell count was associated with a decrease in extent of subclinical atherosclerosis in multiple arterial beds, independent of 10-year CVD risk. Further investigations of associations of CD34+ cell count with subclinical atherosclerosis in asymptomatic individuals could provide mechanistic insights into the atherosclerotic process.
骨髓来源的祖细胞（PCs）可能通过积极修复受损内皮来维持血管健康。本研究旨在确定在无高血压或糖尿病的无症状老派阿米什男性（n = 90）中，外周血CD34 +细胞计数所确定的PC计数是否与心血管疾病（CVD）的10年风险及亚临床动脉粥样硬化指标相关。
通过荧光激活细胞分选获得CD34 +细胞计数，通过电子束计算机断层扫描获得冠状动脉钙化（CAC）情况，并获取CVD危险因素。还对57名男性的子集进行了颈动脉内膜中层厚度（CIMT）测量。在调整10年CVD风险后，CD34 +细胞计数与CAC量（p = 0.03）和CIMT（p < 0.0001）显著相关。自然对数转换后的CD34 +细胞计数每增加1个单位，CAC量估计减少55.2%（95%CI：-77.8%至-9.3%），CIMT估计减少14.3%（95%CI：-20.1%至-8.1%）。
CD34 +细胞计数增加与多个动脉床亚临床动脉粥样硬化程度降低相关，独立于10年CVD风险。对无症状个体中CD34 +细胞计数与亚临床动脉粥样硬化关联的进一步研究可为动脉粥样硬化过程提供机制性见解。
